Abstract: To study the chemical constituents from stems and leaves of Fissistigma retusum. The chemical constituents from F.retusum were separated and purified by silica gel,ODS,Sephadex LH-20 gel column chromatographies,and preparative HPLC.Their structures were identified by physicochemical properties, spectroscopic analysis,as well as comparisons with the literature data.As a result,12 compounds were isolated from 95% ethanol extract from F.retusum, and identified as protocatechuic acid ethyl ester (1),(S)-methoxy-(3,5-dimethoxy-4-hydroxyphenyl) ethanediol (2),2-cis-4-trans-abscisicacid (3),kaempferol-3-O-а-L-arabinopyranoside (4),naringenin (5),aromadendrin (6),eriodictyol (7),(2R,3S)-5,7,3′-tri-methoxy-epicatechin (8),5,6,8-tri-methoxy-7-hydro-xylflavonone (9),syringaresinol (10),epiyangambin (11) and seartemin (12).Compounds 1-12 were isolated from the genus Fissistigma for the first time.

Key words: Fissistigma retusum, chemical constituents, phenolic acids, terpenes, flavonoids, lignans