To explore the anti-tumor effect and the possible mechanisms of HMA against colon cancer cells.The CCK8 methods was used to assay the anti-proliferative activity of HMA.The level of intracellular ROS and GSH was measured by commercial kit.Western blot assay was used to detected the protein expression change of Keap-1/Nrf-2/ARE signaling pathway.The results showed that HMA could significantly inhibit the proliferation of colon cancer cells (P<0.05).Compared to the control group,HMA increased the level of ROS while decreased the GSH content,and inhibited the Keap-1/Nrf-2/HO-1 pathway signaling.In conclusion,HMA could significantly inhibit the proliferation of colon cancer cells by disturbing the redox balance,which was related to inhibiting the Keap-1/Nrf-2/HO-1 signaling pathway.