NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2021, Vol. 33 ›› Issue (2): 268-274.doi: 10.16333/j.1001-6880.2021.2.011

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The anti-proliferative activity and mechanism exploration of 2-hydroxyl group-3-tectoquinone on colon cancer cells

TAN Jia-ni1,2,LIU Wen-hao1,2,SHEN Wei-xing1,2,SUN Dong-dong1,2*,CHENG Hai-bo1,2*   

  1. 1 Translational Medicine Center of Nanjing University of Chinese Medicine,Key Laboratory of Famous Doctors' Proved Recipe Evaluation and Transformation of State Administration of Traditional Chinese Medicine, Provincial Lab of Anticarcinoma Proved Recipe Research and Engineering Industrialization;2 TCM Treatment on Tumor Collaborative Innovation Center of Jiangsu Province,Nanjing 210023,China
  • Online:2021-02-28 Published:2021-03-04

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Abstract:

To explore the anti-tumor effect and the possible mechanisms of HMA against colon cancer cells.The CCK8 methods was used to assay the anti-proliferative activity of HMA.The level of intracellular ROS and GSH was measured by commercial kit.Western blot assay was used to detected the protein expression change of Keap-1/Nrf-2/ARE signaling pathway.The results showed that HMA could significantly inhibit the proliferation of colon cancer cells (P<0.05).Compared to the control group,HMA increased the level of ROS while decreased the GSH content,and inhibited the Keap-1/Nrf-2/HO-1 pathway signaling.In conclusion,HMA could significantly inhibit the proliferation of colon cancer cells by disturbing the redox balance,which was related to inhibiting the Keap-1/Nrf-2/HO-1 signaling pathway.

Key words: HMA, colorectal cancer, oxidative stress, Keap-1/Nrf-2/HO-1 signaling pathway

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