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Research Progress on Arbutin and Arbutin Synthase LIN Kai1, WANG Shi-yu2, GUO Feng-gen1 NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2018, 30 (增刊2): 247-252.   Abstract （509）      PDF（pc） （669KB）（2830）       Knowledge map    Save Arbutin is an important natural product,which has been widely used in medicine,cosmetics,food and other fields.It occured in many plants but its contents were generally low.Arbutin synthase is the key enzyme for arbutin biosynthesis.It can catalyze the reaction of hydroquinone and carbonyl group to arbutin.In order to provide a theoretical reference for the further production and utilization of arbutin,this article reviewed the research progress on arbutin and arbutin synthase from application value,plant resources and biosynthesis pathway of arbutin and the enzymatic properties of arbutin synthase. Related Articles | Metrics | Comments（0）

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Isolation and Purification of Angelica Polysaccharide and Analysis of Its Monosaccharide Composition SUN Hong-guo, ZHANG Man, JI Peng, HUA Yong-li, SONG Zhi-xue, WEI Yan-ming* NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2014, 26 (4): 480-485.   Abstract （1681）      PDF（pc） （621KB）（2351）       Knowledge map    Save Angelica polysaccharide was extracted from Angelica by water extraction and ethanol precipitation.The obtained Angelica polysaccharide was deproteinized with Savage method combined with Papain method.It was then decolorized with H2O2 followed by dialysis.Finally five fractions were obtained by using DEAE-cellulose 52 column chromatography. The fivefractions were identified by Ultraviolet spectroscopy and SephadexG-100.The results showed that they were all single component.The five fractions were further analyzed byinfrared spectrometry,thin-layer chromatography and GC-MS.The results showed that the components of the five polysaccharide were all pectin polysaccharide.WASP-1 might be pyran ring Angelica polysaccharide compound;WASP-2 was β-type pyran ring Angelica polysaccharide compound;WASP-3 was β-type pyran ring and α-type D-pyran ring Angelica polysaccharide compound;WASP-4 was pyran ring Angelica polysaccharide compound;WASP-5 was β-typeAngelica polysaccharide compound,and might contain pyran ring;The GC-MS analysis results showed that sorbose,arabinose,glucose andgalactose were found in WASP-1,WASP-2 and WASP-3;Glucose and galactose were found in WASP-4;Glucose was found in WASP-5. Related Articles | Metrics | Comments（0）

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Chemical Constituents of the Bark of Anthocephalus chinensis XU Xiao-yu, LI Shang-zhen, SONG Qi-shi NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2011, 23 (3): 393-398.   Abstract （5114）      PDF       Knowledge map    Save Ten compounds were isolated and purified from the bark of Anthocephalus chinensis by column chromatography on silica gel, MCI and Sephadex LH-20, and their structures were elucidated as 4-carboxy-3-hydroxy-5-methylphenyl 3-methoxy-4-hydroxy-5-methylbenzoate (1), sitosterol-3-O-(6'-O-palmityl)-β-D-glucoside (2), quinovic acid-3-O-α-L-rhamnopyranoside (3), clethric acid (4), hederagenin (5), uncargenin C (6), morolic acid (7), caffeic acid methyl ester (8), cadambine (9), and 3α-dihydrocadambine (10) by modern spectroscopic methods. Compound 1 was a new phenolic compound, and compounds 2-8 were obtained from Anthocephalus plantsfor the first time. Reference | Related Articles | Metrics | Comments（0）

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Antioxidant Activity of Flavonoids from Branches Leaves and Berry of Hippophae rhamnoides Linn JIAO Yang1,LI Cai-xia1,GAO Hai-ning1,FENG Jiu-hai2* NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2015, 27 (4): 655-660.   DOI: 10.16333/j.1001-6880.2015.04.020 Abstract （1671）      PDF（pc） （695KB）（1507）       Knowledge map    Save Using flavonoids from Hippophae rhamnoides L.berry (ZYCFJ) as reference,the antioxidant activities of flavonoids in branches and leaves of Hippophae rhamnoides L.from Zhangye (ZYCF) and Xinjiang (XJCF) were evaluated by measuring their scavenging activity against DPPH,ABTS,hydroxyl,superoxide anion radicals and reducing Fe3+ and inhibiting nitroso.The results showed that at 50 μg/mL concentration level,the clearance rates of ZYCFJ,ZYCF and XJCF on ABTS+ were 60.8%,42.6%,45.4%,and on DPPH were 95.7%,80.6%.88.6%.When flavonoids concentrations higher than 40 μg/mL,the scavenging activity against DPPH and ABTS+ of ZYCFJ was better than XJCF and ZYCF.At 500 μg/mL concentration level,the scavenging rates of ZYCFJ,ZYCF and XJCF on ·OH were 92.7%, 88.9%, 98.6%,and on O2- were 76.5%,70.6%,82.4%,and inhibition ratio of ZYCFJ,ZYCF and XJCF on nitrite were 98.7%,76.8%,83.7%.In term of the inhibition rates of nitrite and the reduction of Fe3+,ZYCFJ was better than that of ZYCF and XJCF.The scavenging effect of ZYCFJ,ZYCF and XJCF on superoxide anion and hydroxyl free radical were equivalent. Related Articles | Metrics | Comments（0）

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Screening and Identification of Pectinase Producing Strain and Study on Its Pectinaseproducing Conditions YUAN Zhi-hui* NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2013, 25 (12): 1627-1630.   Abstract （1125）      PDF（pc） （476KB）（1284）       Knowledge map    Save The aim of this work was to screen highly pectinase producing bacteria for following laboratorial research and industrial application.The soil sample that is rich in pectin substance was collected from ramie field located in the suburbs of LingLing district for screening of pectinase producing bacteria,and 11 stains were gained by enrichment procedure and screening test successively.Strain HYL6 which produced the highest enzyme activity tested by 3,5dinitrosalicylic acid (DNS) method in 11 strains was identified as Bacillus sp.and its pectinase producing conditions were optimized by onefactor experiments and orthogonal test successively.The optimized conditions were as follows:soluble starch 5 g/L,peptone 15 g/L,pH 7,and shaking fermentation 34 hours at 36 ℃,180 rpm.The activity of pectinase produced by the strain HYL6 under the optimal conditions reached 415 U/mL. Related Articles | Metrics | Comments（0）

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Pharmacological mechanism and network pharmacology research of Huashibaidu formula in treating COVID-19 LAI Qing-lai, LIANG Ai-wu, HE Miao-yi, HUANG Xiao-yu, WU Wei-yong NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2020, 32 (6): 909-919.   DOI: 10.16333/j.1001-6880.2020.6.002 Abstract （365）      PDF（pc） （2104KB）（1282）       Knowledge map    Save This article aims to explore the modern pharmacological study of Huashibaidu formula in treating COVID-19 and apply the network pharmacology method to predict the mechanism of Huashibaidu formula in treating COVID-19.In order to summarize the modern pharmacological study of Huashibaidu formula in the treatment of COVID-19,the literature and clinical reports.were consulted.In order to obtain the main active compounds and corresponding targets of Huashibaidu formula,the TCMSP database was applied.The Gene Cards database was used to obtain the disease targets of COVID-19.The STRING database was used to construct protein interaction networks and the core targets were subjected to GO enrichment and KEGG pathway analysis.Cytoscape3.7.0 software was used to construct compound-target networks.The core compound was molecularly docked with SARS-CoV-2 3CL hydrolase and ACE2 receptor.The modern pharmacological studies have shown that Huashibaidu formula have the effect of anti-inflammatory,anti-viral and immune-modulating.261 traditional Chinese medicine targets,251 disease-related targets and 49 key targets were obtained by the intersection of drugs and disease targets.GO enrichment includes 1 547 biological processes,29 cellular components and 86 molecular functions.KEGG pathway enrichment resulted in 156 pathways related to COVID-19 (P<0.05),involving Kaposis sarcoma-associated herpes virus infection,human cytomegalovirus infection,influenza A,IL-17 pathway,TNF pathway,AGE- RAGE pathway and other pathways.It is predicted that the main core compounds are quercetin,luteolin,kaempferol,begonin,naringenin,β-sitosterol,baicalein,etc.,which play a key role in the entire network.The results of molecular docking showed that quercetin,luteolin,kaempferol had a good combination with 3CL hydrolase and ACE2.This study reveals the characteristics of "Huashibaidu formula " in treating COVID-19 "multi-component,multi-target,multi-channel",which provide a reference for in-depth discussion of the mechanism of action of Huashibaidu Decoction in the treatment of COVID-19. Related Articles | Metrics | Comments（0）

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Identification and Quantification of Phenolic Components of Erigeron breviscapus and Its Derived Drug Breviscapine ZHANG Ren-wei 1*,FAN Xian-er1,LI Lai-wei1,LIN Long-ze2,HARNLY James M2 NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2015, 27 (6): 962-970.   DOI: 10.16333/j.1001-6880.2015.06.005 Abstract （1300）      PDF（pc） （260KB）（1147）       Knowledge map    Save A standardized HPLC-PDA-ESI/MS profiling method was used to identify the phenolic components of Erigeron breviscapus and breviscapine. A general phenolic quantification method was used to quantify the components. As a result,33 hydroxycinnamates and 16 flavonoids were detected in E. breviscapus, a plant used in traditional Chinese medicine for treating cardiocerebral diseases. Eighteen flavonoids were detected in breviscapine,an E. breviscapus derived drug. The hydroxycinnamates were quantified using HPLC-PDA at the detection wavelength of 326 nm and the mole relative response factors (MRRF). Dried chlorogenic acid was used as calibration standard. Similarly,8 apigenins,4 luteolins,3 flavonol 3-O-glycosides,and the 7-O-glucuronides of quercetin and isorhamnetin were quantified using apigenin (at 336 nm) and rutin (at 354 nm) as calibration standards and MRRF value for each compound. This was the first detection of over 10 flavonoids in breviscapine,and the quantification of each phenolic component in E. breviscapus. Related Articles | Metrics | Comments（0）

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Advance on Chemical Compositions and Pharmacological Studies of Semiaquilegia adoxoides XU Ran, XIAO Hai-tao, WANG Jian-ta, TANG Lei* NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2014, 26 (7): 1154-1159.   Abstract （1629）      PDF（pc） （674KB）（1101）       Knowledge map    Save The chemical constituents,pharmacological and clinical effects of the Semiaquilegia adoxoides were reviewed in orde to provide a reference for future research and development of the genus. Related Articles | Metrics | Comments（0）

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Lyngbyatoxin A from Deep South China Sea-Derived Streptomyces sp.SCSIO 5604 TANG Gui-ling1,2, HUANG Hong-bo1, WANG Bo1, TIAN Xin-peng1, JU Jian-hua1*, SONG Yong-xiang1* NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2014, 26 (11): 1767-1770.   Abstract （1229）      PDF（pc） （519KB）（1085）       Knowledge map    Save The strain SCSIO 5604 was identified to be Streptomyces sp.by 16S rRNA gene sequence,which was isolated from a sediment sample collected in South China Sea at E 120° 0.250′ and N 20° 22.971′ at 3536 m depth.Guided by brine shrimp lethality and HPLC-DAD,chemical analysis of the crude extract of the fermentation culture,led to the isolation of an indolelactam compound,namely lyngbyatoxin A.The structure of lyngbyatoxin A was identified based on the HR-ESI-MS, 1H-NMR,13C-NMR spectra and comparing with literature data. Related Articles | Metrics | Comments（0）

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The Extraction Technology of Cannabidiol in Hemp Leaves GAO Bao-chang, SUN Yu-feng, ZHANG Xu, TIAN Yuan, SHI Yu, CUI Bao-yu NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2018, 30 (增刊1): 153-156.   Abstract （620）      PDF（pc） （692KB）（1030）       Knowledge map    Save leaves.The optimized conditions were extract with ethyl alcohol,ultrasound-assisted oscillate for 15 minutes,ratio of sample to solvent 1∶20,extraction 3 times.Meanwhile,the purification process of cannabidiol was also studied.The adsorption and elution capacity of cannabidiol by macroporous adsorption resin were investigated.The optimized macroporous adsorption resin is DM130.The product was characterized by HPLC,LCMS and HNMR.Finally,the cannabidiol of extracting and purification was established. Related Articles | Metrics | Comments（0）

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Compounds from Lycium ruthenicum QI Jing-jing1,2, YAN Yong-ming3, WANG Cheng-xiang4, CHENG Yong-xian1,2,3* NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2018, 30 (3): 345-353.   DOI: 10.16333/j.1001-6880.2018.3.001 Abstract （1199）      PDF（pc） （817KB）（1021）       Knowledge map    Save Twenty two compounds were isolated from the fruits of Lycium ruthenicum Murr. Their structures were identified as 4-p-cumaroyl-α-L-rhamnosyl-(1→6)-β-D-glucose(1),4-p-cumaroyl-α-L-rhamnosyl-(1→6)-α-D-glucose (2),isosalipurposide (3),ethyl caffeate (4),trans-ferulic acid (5),quercetin 3-O-β-D-rutinoside (6),kaempferol-3-O-rutinoside (7),isorhamnetin 3-rutinoside (8),kaempferol 3-rutinoside-4′-glucoside (9),naringenin 7-O-β--D-glucopyranoside (10),ampelopsin 4′-O-β--D-glucopyranoside (11),helichrysin A (12),naringenin (13),quercetin (14),scopoletin (15),evofolin B (16),(+)-isolariciresinol (17),methyl-2,4-dihydroxy-5-methoxybenzoate (18),3-O-methylgallic acid (19),4-acetyl-2,6-dimethoxyphenol (20),acetovanillone (21) and methyl benzoate (22),respectively,by spectroscopic methods.Compound 1 is a new substance.In addition,compounds 2,3,9,11,12,and 16-19 were isolated from the genus Lycium for the first time. Related Articles | Metrics | Comments（0）

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Cladosperanol A,a New Dimeric Tetralone from Marine-derived Fungus Cladosporium perangustum FS62 FAN Zhen, SUN Zhang-hua, CHEN Yu-chan, LI Hao-hua, ZHANG Wei-min NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2016, 28 (4): 487-489.   Abstract （937）      PDF（pc） （585KB）（1013）       Knowledge map    Save A new compound (1) was isolated from the fermentation broth extract of marine fungal strain Cladosporium perangustum FS62 by various chromatographic methods such as silica gel,reverse silica gel,Sephadex LH20,HPLC, etc. On the basis of detailed spectroscopic analysis (HR-ESI-MS,1D NMR,2D NMR,CD),1 was elucidated as a new dimeric tetralone with the structure of (4S,4′S)-4,8,8(trihydroxy-2,3,2′,3′- tetrahydro-［4′,5-binaphthalene］-1,1′(4H,4′H)-dione,named as cladosperanol A. Related Articles | Metrics | Comments（0）

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A New DrimaneSesquiterpenoid from Cultures of Fungus Psathyrellacandolleana LI Xue-mei1,2, YIN Xia1,3, LI Zheng-hui1, FENG Tao1*, LIU Ji-kai1* NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2015, 27 (7): 1131-1133.   DOI: 10.16333/j.1001-6880.2015.07.001 Abstract （1570）      PDF（pc） （136KB）（988）       Knowledge map    Save A new drimanesesquiterpenoid15-hydroxydrimenol (1), together with a known germacranesesquiterpenoid1α-hydroxy-bisabola-2,10-dien-4-one (2),was isolated from cultures of fungus Psathyrellacandolleana. The new compound 1 was elucidated on the basis of extensive spectroscopic methods,while the known compound 2 was identified by comparison of spectroscopic data with those reported in the literature. Related Articles | Metrics | Comments（0）

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Anti-inflammatory Activity of Forsythia suspensa Extract on Human Airway Epithelial Cells Inflammation Model LIU Jian-zhou,ZHANG Li-wei* NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2015, 27 (7): 1248-1253.   DOI: 10.16333/j.1001-6880.2015.07.023 Abstract （1360）      PDF（pc） （945KB）（975）       Knowledge map    Save To investigate the anti-inflammatory activities of Forsythia suspensa extract (FSE) with emphasis on excretion of inflammatory cytokines by LPS-induced BEAS-2B inflammation model.BEAS-2B cell viability was detected by MTT method in order to screen out the optimal drug dose of F.suspensa ethanol extracts (FSEE),60% forsythiaside A (60% FTA) extracts and 90% forsythiaside A (90% FTA) extracts;The morphology of BEAS-2B cells treated with FSE were observed by microscope; NO content of cell culture supernatant was determined by Griess Reagent method;Intracellular reactive oxygen species (ROS) was analyzed by flow cytometry.Toxical experimental results revealed that FSE did not show toxic reaction to BEAS-2B cell at concentration of less than 128 μg/mL;while both 60% FTA and 90% FTA showed toxicity at the concentration between 128 and 256 μg/mL.Anti-inflammatory results showed that all FSE can inhibit the secretion of NO (P<0.01) at concentration 25,50,and 100 μg/mL,and 90% FTA extracts exhibited a dose-dependent manner;The elevated level of intracellular ROS in LPSstimulated BEAS-2B cells was significantly diminished by all FSE in a dose-effect manner (P<0.05).In addition,the therapeutic effect of FSEE,60% FTA and 90% FTA increased gradually.It was concluded that the 90% FTA extracts were the main active fraction of anti-inflammatory effect and forsythiaside A was possibly the active ingredient.The results presented in this study will be helpful for the further study of F.suspensa on anti-inflammatory activity. Related Articles | Metrics | Comments（0）

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A New Lactone Aldehyde Compound Isolated from Secondary Metabolites of Marine Fungus Penicillium griseofulvum XU Yan,TIAN Sha-sha,ZHU Hua-jie* NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2015, 27 (4): 559-561.   DOI: 10.16333/j.1001-6880.2015.04.001 Abstract （1415）      PDF（pc） （284KB）（970）       Knowledge map    Save Three chemical constituents were separated and purified from marine-derived fungus Penicillium griseofulvum by silica gel column chromatography,semipreparative HPLC,ODS column chromatography and Sephadex LH-20 column chromatography.On the basis of spectral data (1D NMR,2D NMR and high resolution MS),their structures were elucidated as 2-(6-hydroxy-5,7-dimethylbenzofuranone-4-yl)acetaldehyde (1),terephthalic acid bis(2-ethyl-hexyl) ester (2) and bis(2ethylhexyl) adipate (3).Compound 1 was a new compound. Related Articles | Metrics | Comments（0）

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Mechanism of Huanglian Jiedu Decoction in treating vascular dementia based on network pharmacology and experimental verification ZHANG Yun-hui, YANG Meng-lin, WU Da-hua, LIU Xia, YANG Kun, LI Xiang NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2023, 35 (3): 509-518.   DOI: 10.16333/j.1001-6880.2023.3.017 Abstract （448）      PDF（pc） （2457KB）（932）       Knowledge map    Save To study the mechanism of Huanglian Jiedu Decoction in the treatment of vascular dementia(VD) by network pharmacology.Searched the effective ingredients and related targets of Huanglian Jiedu Decoction by the TCMSP database,then obtained the targets of VD through the DisGeNET and GeneCards database.Constructed protein interaction network map by String online database,and to performed GO and KEGG enrichment analysis of key targets by R language.Induction of PC12 cell injury by adopted glutamate as VD cell model.Detection of cell viability by the MTT assay,and reactive superoxide dismutase (SOD),glutathione peroxidase(GSH-Px),malondialdehyde(MDA) content by using Kits.ELISA and Western blot were used to verify the main biological processes and signaling pathways as predicted by network pharmacology.A total of 63 active ingredients of Huanglian Jiedu Decoction were screened,216 corresponding targets,105 targets related to VD,the top 20 KEGG related signaling pathways and GO analysis of the top 20 biological processes.Cell experiments confirmed that Huanglian Jiedu Decoction effectively increased the survival rate of glutamate-induced PC12 cells,inhibited the inflammatory response and oxidative stress response,reduced the protein expression of JNK,p-JNK,FoxO1,LC3Ⅱ/Ⅰ,Beclin1,Atg7,Caspase-3,Caspase-9,increased the protein expression of p-FoxO1 and P62.Huanglian Jiedu Decoction had a therapeutic effect on VD by inhibited the JNK/FoxO1 pathway,thereby inhibited inflammation,oxidative stress,autophagy and apoptosis. Related Articles | Metrics | Comments（0）

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Isolation,Purification and Determination of Yukovanol in Desmodium caudatum (Thunb.) DC LU Guo-shou1, YE Yong2*, LU Wen-jie1,3, HUANG Zhou-feng1, LAN Yan-ni2, ZENG Fan-qiang2, HUANG Wei-cheng2 NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2014, 26 (9): 1450-1453.   Abstract （1418）      PDF（pc） （446KB）（929）       Knowledge map    Save A flavonoid was isolated from Desmodium caudatum (Thunb.) DC by silica gel column chromatography. The structure of the isolated compound was elucidated on the basis of spectral characteristics and identified as yukovanol. The content of yukovanol in D.caudatum was determined by HPLC.The chromatographic separation were carried out using the following condition:Agilent ZORBAX SB-C18 column (250 mm × 4.6 mm,5 μm) was used with methanol-0.2% phosphoric acid (70:30) as mobile phases.The flow rate was 1.0 mL/min.The UV detection wavelength was 270 nm and the column temperature was 35 oC.The results showed that the linear range of yukovanol were 0.011-0.242 μg,the yukovanol average recovery as 99.76%,RSD = 1.42% (n=6).Distinct differences were found among D.caudatum samples produced in different places. Related Articles | Metrics | Comments（0）

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Extraction and Purification of Ginkgo biloba Flavonoids Using ［C8 mim］［N(CN)2］/(NH4)2SO4 Aqueous Two-Phase System XU Xue-lei, LI Fen-fang*, TAN Zhi-jian NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2014, 26 (6): 830-833.   Abstract （1290）      PDF（pc） （415KB）（916）       Knowledge map    Save A new method for the extraction and purification of Ginkgo biloba flavonoids in an aqueous two-phase system (ATPS) formed from hydrophilic ionic liquid ［C8 mim］［N(CN)2］(1-octyl-3-methylimidalium dicyanamide) and phase-forming salt (NH4)2SO4 was studied in this study.The effects of concentration of salt, temperature of system,pH value,amount of electrolyte of NaCl on extraction efficiency were investigated.The parameters influencing the recovery efficiency of (NH4)2SO4 were investigated.(NH4)2SO4 can be recovered from salt solution with higher recovery efficiency.When the ATPS contained 18.52% ［C8 mim］［N(CN)2］and 25.93% (NH4)2SO4,and 1.5 mmol NaCl was added at 298.15 K with pH being not adjusted,the extraction efficiency was 96.73%.IL/salt ATPS had higher extraction efficiency and lower viscosity than other ATPSs.When adding 2 volumes of methanol, (NH4)2SO4 can be recovered 90.54%.ATPS based on ［C8 mim］［N(CN)2］/(NH4)2SO4 was a good method on extraction and purification of Ginkgo biloba flavonoids. Related Articles | Metrics | Comments（0）

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Chemical Constituents and Their Toxic Activity from the Endophytic Fungus Phomopsissp. KY-12, Isolated from Pleioblastus amarus GUO Fang1, YANG Sheng-xiang1,2*, LIU Li1,2*, WANG Yong2 NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2014, 26 (9): 1389-1392.   Abstract （1461）      PDF（pc） （348KB）（864）       Knowledge map    Save To study the chemical constituents and their toxic activity from the endophytic fungus Phomopsis sp. KY-12, isolated from Pleioblastus amarus. The compounds were isolated and purified by means of chromatographic techniques and their structures were identified on the basis of spectral features. Cytotoxic activity was performed by the brine shrimp (Artemiasalina) larvae bioassay.13known compounds including 9Cadinanesesquiterpenoids, 1 azaphilone and 3 steroids,named 3,12-dihydroxycalamenene (1), 3,9,12-trihydroxycalamenene (2), indicumolide C (3), agripilol C (4), 2,15-tihydroxycalamenene (5), dysodensiol D (6), bombamalones D (7), 8-formyl-7-hydroxyl-5-isopropyl-2-methoxy-1,4-naphthoquinone (8), strobilol A (9), Pyrenocine J (10), 3β, 5α-Dihydroxy-6β-methoxyergosta-7,22-dien (11), ergosterol (12) and ergosterol peroxide (13),were isolated from the organic extract of fermentation broths of the endophytic fungus, Phomopsis sp. KY-12. Compounds 3-9 were first isolated from the Phomopsis genus.Compounds 1-8 showed moderate active against the brine shrimp larvae,compound 9 indicated significant growth inhibitory activity against the brine shrimp (Artemias alina) larvae. Related Articles | Metrics | Comments（0）

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Identification of Glucosinolates and Organic Acids in Thlaspi arvense L.by LC-ESI-MS/MS YU Jin-ying1,2, WANG Yun-hong2, LIU Guo-qiang3, WANG Bin-hao3, PAN Zheng4, YANG Rong-ping1,2* NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2015, 27 (1): 67-72.   DOI: 10.16333/j.1001-6880.2015.01.014 Abstract （1217）      PDF（pc） （551KB）（846）       Knowledge map    Save The objective of this study was to identify the main components of Thlaspi arvense L.by LC-ESI-MS/MS.The separation was performed on Thermo Scientific Syncronis C18 column (150mm×2.1mm,3 μm),with a mobile phase consisting of 0.1% formic acid in water (A) and acetonitrile (B) for gradient elution.The analysis was performed in positive and negative ion mode using full scan mode with ESI source.Ten glucosinolates and eleven organic acids were well separated and identified from the extracts of T.arvense.9 glucosinolates were identified for the first time from T.arvense including six pairs of structural isomers.The new method was accurate,rapid and effective.It laid the foundation for further research of T.arvense. Related Articles | Metrics | Comments（0）

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Review on Anti-adhesion Activity of Tetramethylpyrazine YAN Shuai,LI Wen-lin, ZENG Li* NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2015, 27 (2): 367-371.   DOI: 10.16333/j.1001-6880.2015.02.033 Abstract （1079）      PDF（pc） （638KB）（837）       Knowledge map    Save Recent studies of anti-adhesion mechanism of tetramethylpyrazine were reviewed to provide reference for clinical study and new drug development.It was found that the anti-adhesion mechanism of tetramethylpyrazine involved multi-targets and multi-links such as the inhibition of proliferation of collagen,over-expression of adhesion related factors,and reduction of the concentration of white blood cells and fibrin in plasma.In future,more clinical evidence was still required,and the basic research of tetramethylpyrazine should be focused on the biomaterial development. Related Articles | Metrics | Comments（0）

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Chemical Constituents of Enzymatic Hydrolysate of Total Ginsenoside Extract of Panax quinquefolium JIN Jian-ming 1,2*, LI You-hai2, ZHANG Hai-lun1, LIANG Chao-ning3, TANG Shuang-yan3 NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2014, 26 (10): 1552-1556.   Abstract （1469）      PDF（pc） （414KB）（835）       Knowledge map    Save The total ginsenoside extract of Panax quinquefolium  was hydrolyzed by β-glycosidase.HPLC analysis indicated that ginsenosides Rb1,Rd,Rc and Rb2 were completely hydrolyzed.Seven ginsenosides were isolated from the hydrolysate,and identified as ginsenoside compound K (1),ginsenoside Mc (2),ginsenoside Rg1 (3),ginsenoside Rg2 (4),ginsenoside Re (5),ginsenoside F1 (6) and pseudoginsenoside F11 (7),respectively.The protopanaxdiol type ginsenosides in the total ginsenoside extract of P.quinquefolium were converted into ginsenoside compound K and ginsenoside Mc by the β-glycosidase. Related Articles | Metrics | Comments（0）

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Study on Combined Extraction of Alkaloids and Polysaccharides from Dendrobium officinale YUAN Lin1, LI Jiao1, RONG Yong-hai1, TONG Zhan-qi2, RONG Long1* NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2015, 27 (1): 179-184.   DOI: 10.16333/j.1001-6880.2015.01.036 Abstract （1484）      PDF（pc） （639KB）（818）       Knowledge map    Save Dendrobium officinale is a kind of traditional Chinese medicine.It contains a variety of active ingredients,such as alkaloids and polysaccharides.In this study,the flash extraction technology and enzymatic hydrolysis were used to extract alkaloid,joined and free polysaccharide from D.officinale.The enzymatic hydrolysis conditions were optimized using a series of one-factor-at-a-time experiments.The results showed that the optimal extraction conditions were as follows:1:30 material/solvent ratio,32 U/g cellulose,39200 U/g papain and 2.5 h hydrolysis time.The extraction yields of alkaloid,joined and free polysaccharide were:0.136%,4.2% and 19.3%,respectively.The purities were:78.2%,81.3% and 90.6%.The molecular weights of joint and free polysaccharides were 2373 Da and 17987 Da,which were determined by GPC.This study developed a combined extraction process of alkaloids and polysaccharides from D.officinale.The process was efficient,convenient and suitable for industrial production. Related Articles | Metrics | Comments（0）

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A New Phenolic Dimer from Ganoderma lucidum MIAO Jin-hua1,2,YAN Yong-ming2, WANG Xin-long1,2, CHENG Yong-xian2* NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2014, 26 (10): 1545-1547.   Abstract （1270）      PDF（pc） （272KB）（809）       Knowledge map    Save Six compounds including one new phenolic dimer were isolated fromGanoderma lucidum by various chromatographic techniques.Their structures were identified as (±) ganolucidin (1), 2-hydroxy-5-ethoxy benzoic acid (2), 2-methyl-6-hydroxychromogen (3), 2,5-dihydroxyacetophenone (4), methyl 2,5-dihydroxybenzoate (5) and 6-hydroxychromogen (6),respectively,by various spectroscopic methods. Related Articles | Metrics | Comments（0）

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Preparation,Characterization and Application of Ginsenoside Rg1bonded Silica Gel Stationary Phase(Ⅱ) GU Xiao-juan, HAN Ya-qiong, LI Xin-yi, SHEN Bao-chun* NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2014, 26 (6): 824-829.   Abstract （1220）      PDF（pc） （681KB）（808）       Knowledge map    Save A new ginsenoside Rg1-bonded silica gel stationary phase (250 mm×4.6 mm,5 μm) was prepared by one-pot reaction methodusing 1,6-diisocyanate as spacer arm.The characterization of the new self-made ginsenoside Rg1-bonded silica gel stationary was done by elemental analysis,thermal analysis and scanning electron microscope(SEM).The HPLC fingerprint of Active Pharmaceutical Ingredient (API) of Panax notoginseng saponins (PNS) on the ginsenoside Rg1-bonded silica gel stationary phase was established.The chromatographic fingerprints of 10 batches PNS API were determined on the self-made ginsenoside Rg1-bonded silica gel column.The mobile phase consisted of acetonitrile and water with gradient elution mode.The detection wavelength was 203 nm,the column oven temperature was maintained at 25 ℃,the flow rate was 0.8 mL/min and the injection volume was 10 μL.Based on the peak of ginsenoside Re,20 common peaks were selected,the similarity between 10 batches of samples were within 0.882-0.999.This study provided a new idea for natural products analysis using ginsenoside Rg1-bonded silica gel stationary phase. Related Articles | Metrics | Comments（0）

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Purification and Analysis of Litchi Pericarp Oligomeric Procyanidins LI Shu-yi1,2, WU Qian2, SUI Yong2, SUN Zhi-da2* NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2015, 27 (1): 11-17.   DOI: 10.16333/j.1001-6880.2015.01.002 Abstract （1539）      PDF（pc） （698KB）（786）       Knowledge map    Save In order to obtain Atype oligomeric procyanidins from litchi pericarp with higher purity,C18 silica gel column was used to optimize the traditional preparation process,which was purified and enriched by using AB-8 macroporous resin.According to the component analysis,HPLC and LC-MS identification of litchi pericarp oligomeric procyanidins (LPOPC) prepared by C18 silica gel,the total phenol content of LPOPC was up to 940±123 mg/g,in which (-)-epicatechin monomer,A-type procyanidin dimer and trimer played an important role,occupying 44.86%,36.02% and 9.86%,respectively.The average polymerization degree of the new LPOPC was about 1.62.Consequently,the known A-type procyanidin oligomers,such as procyanidin A2 and trimer,epicatechin (4β→8,2β→O→7)-epicatechin-(4β→8)-epicatechin,can be isolated from the LPOPC more efficiently by Toyopearl HW-40s column.In addition,the isobologram analysis suggested that the purified A-type procyanidin dimer and trimer all possessed strong radical scavenging capacities,and showed synergistic antioxidant effect. Related Articles | Metrics | Comments（0）

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Chemical Constituents from Ganoderma applanatum and Absolute Configuration Determination of New Compounds BU Qing-hua1,2#,LUO Qi2#,CHENG Yong-xian2* NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2016, 28 (5): 650-654.   DOI: 10.16333/j.1001-6880.2016.5.002 Abstract （1380）      PDF（pc） （540KB）（776）       Knowledge map    Save Eight compounds including two new compounds,namely shushenes A (1) and B (2),were isolated from the fruiting bodies of Ganoderma applanatum. Their structures were identified by means of spectroscopic methods.The structures of the known compounds are lucidulactone A (3),2,3-dihydroxy-1-(4-hydroxy3,5-dimethoxyphenyl)-1-propanone (4),6-hydroxy-4-H-chromen-4-one (5),1-(4-ethylphenyl)-1,2-ethanediol (6),1-(2-ethylphenyl)-1,2-ethanediol (7) and indole-3-carboxaldehyde (8),which were all isolated from this species for the first time.Of note,compounds 1 and 2 were isolated as racemic mixtures,chiral separation followed by computational methods allowed their absolute configuration assignment.In addition,inhibition of compounds 1 and 2 on fibronectin expression was tested by using an enzyme-linked immunosorbent assay (ELISA). Related Articles | Metrics | Comments（0）

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Chemical Constituents from the Fruits of Capparis masaikai LIAO Jin-hua1, HU Xu-jia1*, YUAN Chun-mao2, HE Hong-ping2, DI Ying-tong2* NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2014, 26 (11): 1780-1784.   Abstract （2025）      PDF（pc） （207KB）（775）       Knowledge map    Save Ten compounds were isolated and purified by silica gel column chromatography and Sephadex LH-20 from the fruits of Capparis masaikai.Their structures were identified as medioresinol (1), erythro-guaiacylglycerol-β-O-4′-sinapyl ether (2),hedyotol C (3),hedyotisol A (4),hedyotisol B (5),ozoroalide (6),5α,6α-epoxy-3β-hydroxyergosta-22-ene-7-one (7),coniferyl aldehyde (8),3-hydroxy-5-(p-hydroxyphenyl) pentanoic acid (9) and β-hydroxypropiovanillone (10) by physicochemical properties and spectral data.The structural types of isolated compounds include lignans,macrocyclic lactone,sterols and phenols.Compounds 1-10 were isolated from this plant for the first time,among of them,compounds 1-7 were firstly reported from the genus Capparis. Related Articles | Metrics | Comments（0）

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Prodrug Decision of Novel Genistein Sulfonate and Its Bioavailability in Rats PENG You, LIU Xin-qiang, DENG Ze-yuan NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2012, 23 (1): 35-41.   DOI: A Abstract （1911）      PDF（pc） （221KB）（774）       Knowledge map    Save The oral bioavailability of genistein (GE) in its benzensulfonate prodrug was studied in search for new drug or food functional ingredient. The plasma were collected at different points of time after the intragastric or intravenous administration of genistein benzensulfonate (GB) 40 mg/kg to rats. The GB and GE contents in plasma were determined by high performance liquid chromatography (HPLC). The compartment model was fitted and pharma cokinetic parameters were calculated by DAS 2.1.1. The results indicated that the dynamic processes of GE was consistent with two compartment model after intragastric or intravenous administration of GB prodrug to rats. The relative oral bioavailability of GE in prodrug GB was 110.9%. In conclusion, the above results demonstrated that the oral bioavailability of GE in prodrug had been improved remarkably. Related Articles | Metrics | Comments（0）

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Effect of Magnesium Cantharidate on the Apoptosis of Subcutaneous Transplantation Tumor from Human Hepatoma Cells SMMC-7721 in Nude Mice YAN Rong1, LIU Yun2, ZHU Xin-ting1, YI Xiao-fei1, LIU Liu1, ZHANG Yun-hui3, LI Xiao-fei1* NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2015, 27 (2): 236-240.   DOI: 10.16333/j.1001-6880.2015.02.008 Abstract （1454）      PDF（pc） （1188KB）（772）       Knowledge map    Save The model of subcutaneous transplantation tumor from human hepatoma cells SMMC-7721 in nude mice was established.Each nude mice was injected with magnesium cantharidate (6.26×10-5 mmol) around the tumor in experimental group,while the same volume of sterile saline was injected as control group.After administration for 22 days,the effect of magnesium cantharidate on the proliferation of subcutaneous transplantation tumor was observed.On the basis of that,the histomorphologic characteristics of subcutaneous transplantation tumor in nude mice was observed by HE staining.HE staining showed that the volume of transplantation tumor cells in magnesium cantharidate group became smaller,cytoplasm pyknosis,acidophilic degeneration,nuclear pyknosis and fragmentation.The ultra-structural changes of subcutaneous transplantation tumor in nude mice was observed by transmission electron microscopy.The results showed that the nuclear membrane of transplantation tumor in magnesium cantharidate group disappeared,chromatin gathered together,etc.The expression level of bcl-2 and bax was detected by Two-step immunohistochemistry.The results showed that the expression of bcl-2 in magnesium cantharidate group was obviously lower,but the expression of bax was higher than that of control group (P<0.05).The study indicated that magnesium cantharidate can inhibit the proliferation of subcutaneous transplantation tumor from human hepatoma cells in nude mice and induce the apoptosis of transplantation tumor cells.The mechanism may be related with the up-regulation of the expression of bax and down-regulation of the expression of bcl-2. Related Articles | Metrics | Comments（0）

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Resveratrol Oligomers from Nardostachys chinensis Batal. WANG Zhong-ping1,2, CHEN Ying-peng1,2, LIANG Shuang 1,2, WANG Juan 1,2, ZHENG Hong-hong 1,2, WU Hong-hua 1,2* NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2014, 26 (10): 1548-1551.   Abstract （1857）      PDF（pc） （355KB）（771）       Knowledge map    Save Five resveratrol oligomers were isolated from the 70% alcohol extracts of the dried roots and rhizomes of Nardostachys chinensis Batal..On the basis of spectroscopic data and physicochemical properties,their chemical structures were identified as α-viniferin (1),kobophenol A (2),ampelopsin A (3),isohopeaphenol (4) and grandiphenol A (5).This is the first report of resveratrol oligomers isolated from NardostachysDC.. Related Articles | Metrics | Comments（0）

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Determination of Amino Acids in Rhubarb Seeds with DNFB Pre-column Derivatization by Reversed-phase High Performance Liquid Chromatography LIU He-chun1,2, XU Wen-hua1, SHEN Jian-wei1, SONG Wen-zhu1, ZANG Li-li3, ZHOU Guo-ying1* NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2014, 26 (7): 1056-1061.   Abstract （1509）      PDF（pc） （575KB）（766）       Knowledge map    Save The aim of the study was to determine the contents of amino acids in rhubarb seeds with pre-column derivatization by reversed-phase high-performance liquid chromatography (RP-HPLC). Amino acids extracted by the method of hydrolysis in 6 mol/L HCl were derivatized by 2,4-dinitrofluorobenzene (DNFB) and analyzed by RP-HPLC with gradient elution.The chromatographic separation was performed on a Gemimi-NX C18 (4.6 mm × 250 mm,5 μm) column with 0.1 M aqueous sodium acetate solution (A) and acetonitrile-water (1:1) (B) as mobile phases.The column temperature was maintained at 37 °C,and the detection wavelength was set at 360 nm.The results showed that the three genuine rhubarb seed contained 17 kinds of amino acids,11.30% to 19.26% of the total content.The experimental results proved that the developed RP-HPLC method was sensitive,accurate and repeatable. Related Articles | Metrics | Comments（0）

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Review on Pancreatic Lipase Inhibitors Derived from Plants LI Qiang,REN Hong*, WANG You-sheng NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2015, 27 (2): 360-366.   DOI: 10.16333/j.1001-6880.2015.02.032 Abstract （1381）      PDF（pc） （646KB）（760）       Knowledge map    Save Lipase inhibitors can reduce the digestion and absorption of dietary lipids by inhibiting pancreatic lipase activity,achieving the purpose of the control and treatment of obesity.Plantderived natural products have a lot of virtues,such as a wide range of sources,structural diversity,highly specificity and low toxicity.Therefore,screening of safe and effective lipase inhibitors derived from plant become a hot topic.This paper reviewed both the methods of determination of lipase inhibiting activity and the lipase inhibitors derived from plants based on the chemical structure. Related Articles | Metrics | Comments（0）

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Effects of Acanthopanax senticosus Polysaccharide on Signal Transduction of Peripheral Lymphocyte of Weaning Piglets HAN Jie,BIAN Lian-quan*,LIU Xian-jun,ZHANG Fei NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2014, 26 (10): 1589-1592.   Abstract （1357）      PDF（pc） （450KB）（752）       Knowledge map    Save The experiment concerning in vitro effect of Acanthopanax senticosus polysaccharide (ASPS) on signal transduction of peripheral lymphocyte was conducted to investigate the mechanism of immunoregulation effect of ASPS.Peripheral blood lymphocyte suspension was prepared,and T,B cells of which were separated and co-incubated with or without various concentrations of ASPS (40,80,160,320 μg/mL) separately to determine lymphocyte proliferation measured by MTT assay.Anti-TLR4 was designed to discuss the lymphocyte receptor.The results showed that ASPS significantly improved the T cell proliferation and the concentration of IL-2 secreted by lymphocyte (P<0.05),but had no significant effect on the B cell proliferation and the concentration of IL-4 (P>0.05).ASPS induced the production of TNF-α,NO,iNOS as well as NF-κB secreted by lymphocyte when TLR4 antibody was not supplemented in culture solution (P<0.05),and these indices were not affected when TLR4 antibody was added in the culture solution (P>0.05).These results suggested that T lymphocyte probably as one of the direct target cells was mediated by ASPS through TLR4/NF-κB signaling pathway. Related Articles | Metrics | Comments（0）

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Supermolecular Inclusion Property of Methylated-β-cyclodextrin with Chrysophanol and Its in vitro Release Behavior WU Zhen1, LI Hong2,3, CHEN Gang1, SHI Xuan1, TAN Hong-jun1* NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2014, 26 (8): 1174-1177.   Abstract （1246）      PDF（pc） （525KB）（750）       Knowledge map    Save To improve the solubility of chrysophanol (Chr),the inclusion complex of Chr-methylated-β-cyclodextrin (M-β-CD) prepared by electromagnetic stirring and spray drying methods (ES) and its supermolecular inclusion property and in vitro release behavior were investigated.Under different temperatures,phase solubility diagrams showed the characteristics of AL type.Under the temperatures of 25, 35 and 45 oC,the supermolecular inclusion constants were 6.56×102, 7.85×102 and 1.09×103 L/mol,respectively.For themodymamic parameters determination, △H0 = 20.09 kJ/mol;△S0 = 121.11 J/(mol·K);△G0= 16.01, 17.21 and 18.42 kJ/mol.The supermolecular complex of M-β-CD with Chr can be formed spontaneously in 1:1 molar ratio.The whole inclusion process was characterized by the entropy driven process.The cumulative release rate of Chr-M-β-CD complex was significant higher than Chr alone and their physical mixture.Chr-M-β-CD complex were sound in physicochemical and release property.It was simple to prepare and can be used as ideal drug delivery system for Chr. Related Articles | Metrics | Comments（0）

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Network pharmacology-based study on material basis and mechanism of Qingfei Paidu Decoction against COVID-19 XU Tian-fu, HE Cheng-gong, YANG Kun NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2020, 32 (6): 901-908.   DOI: 10.16333/j.1001-6880.2020.6.001 Abstract （590）      PDF（pc） （1696KB）（750）       Knowledge map    Save This study used the method of network pharmacology to study the multi-component，multi-target and multi-pathway mechanism of Qingfei Paidu Decoction in the treatment of COVID-19，in order to provide basis for related basic research and clinical application.First of all，according to the screening conditions of OB ≥ 30% and DL ≥ 0.18，302 active components and 148 related targets in Qingfei Paidu Decoction were screened by TCMSP database，screening of 362 COVID-19 -related targets by GeneCards database，23 intersection targets were obtained by Venn analysis.Then 10 significant effective compounds and 5 key targets were obtained by using the CentiScaPe plug-in of Cytoscape software，Further construct the network topology diagram.The results of molecular docking of significant effective compounds and key targets show that the binding ability and interaction ability between molecules are strong.Finally，the GO and KEGG pathway enrichment analysis of the key targets were done through the ClusterProfiler package of R software，key compounds such as quercetin，luteolin，naringin，kaempferol and baicalein have antitussive，expectorant，anti-inflammatory and antiviral effects to varying degrees，the key targets are mainly concentrated in 144 related signal pathways such as IL-17，Tuberculosis，TNF，MAPK，Th17，Pertussis，etc.It involves 28 biological functions such as phosphatase binding，MAP kinase activity and cytokine receptor binding to regulate metabolism，immune regulation，inflammation and other physiological processes.According to the above results，it is considered that the active components of Qingfei Paidu decoction have multi-target and multi-pathway regulating effect on the treatment of COVID-19. Related Articles | Metrics | Comments（0）

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Study on Antioxidant and Antibacterial Activities of Carnosic Acid XIA Tian-juan1, BI Liang-wu1,2*, ZHAO Zhen-dong1,2, XING Ya-li1, CHENG Xian1 NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2015, 27 (1): 35-40.   DOI: 10.16333/j.1001-6880.2015.01.007 Abstract （1560）      PDF（pc） （670KB）（748）       Knowledge map    Save The antioxidant and antibacterial activities of carnosic acid were studied by radical scavenging effect,reducing ability and tablet dilution methods.The results showed that,the antioxidant activity of carnosic acid was significantly higher than that of synthetic antioxidants PG,BHT,BHA,VE under the same concentration,while slightly lower than THBQ.At the same time,IC50s of scavenging DPPH and ABTS radical of carnosic acid were 2.53 μg/mL and 51.58 μg/mL,respectively.Antibacterial tests indicated that carnosic acid had broad-spectrum of antibacterial activity.Especially for Escherichia coli and Pseudomonas aeruginosa, the MICs of carnosic acid were 4 and <2 μg/mL,better than that of bromogeramine,sodium ampicillin and erythromycin.Moreover, for Klebsiella pneumonia, carnosic acid showed the same inhibitory effect with erythromycin,better than bromogeramine and sodium ampicillin. Related Articles | Metrics | Comments（0）

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Isolation and Characterisation of Enterocins from Marine Streptomyces sp.SCSIO 11863 Isolated from South China Sea Kumar Saurav, ZHANG Qing-bo, LI Su-mei, ZHANG Wen-jun, TIAN Xin-peng, LI Hui-xian, ZHANG Hai-bo* NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2014, 26 (8): 1216-1220.   Abstract （1231）      PDF（pc） （245KB）（743）       Knowledge map    Save An actinomycetes strain SCSIO 11863 with strong antibacterial activity was isolated from marine sediment of the South China Sea.Based on the phenotypic and phylogenetic analysis,the strain was identified as a member of Streptomyces and designated as Streptomyces sp.SCSIO 11863 (KC904267).16S rDNA sequence showed 99% similarity with Streptomyces albogriseolus strain ABRIINW EA1145 (GQ925802).Bioactivity guided extraction with ethyl acetate resulted in the isolation of two analogue compounds.Structures were elucidated with detailed analyses of NMR and MS spectra and identified as Enterocin (1) and 5-deoxyenterocin (2). Related Articles | Metrics | Comments（0）

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Flavonoid Components of Oxytropis myriophylla (Pall.) DC. MENG Gen-xiao1,2,WANG Qing-hu2,GUO Yu-hai1,AO Wuliji2* NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2014, 26 (10): 1614-1617.   Abstract （1410）      PDF（pc） （321KB）（739）       Knowledge map    Save Ten flavonoids were separated and purified from Oxytropis myriophylla (Pall.) DC.by silica gel column chromatography,Sephadex LH-20 and preparation HPLC,etc. Based on the spectral analysis,their structures were elucidated as 4,4′-dimethoxy-2′-hydroxychalcone (1),2′,4′-dihydroxy-4-methoxychalcone (2), 7,8-dihydroxyflavonone (3), 4,2′,4′-trihydroxychalcone (4),2′,4′-ihydroxydihydrochalcone (5),4′-hydroxy flavonone-7-O-β-D-glucoside (6),2′,4′-dihydroxychalcone (7), apigenin (8), apigenin-7-O-β-D-glucuronide (9) and 3′,7-dihydroxy-2′,4′-dimethoxyisoflavan (10).Compounds 1-9 were isolated from this plant for the first time. Related Articles | Metrics | Comments（0）

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Preparative Isolation and Purification of Four Phenanthrenes from Juncus effusus by HSCCC LI Gui-yun, CHEN Ze-man, WANG Xiao-hong, HUANG Jian-mei* NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2014, 26 (8): 1248-1251.   Abstract （1344）      PDF（pc） （476KB）（738）       Knowledge map    Save This study is to establish the high-speed counter-current chromatography method for purification of effusol,dehydroeffusol,juncusol,dehydrojuncusol from Juncus effusus L. A two-phase solvent system was composed of cyclohexane-ethyl acetate-methanol-water(5:3:5:3),The upper phase was stationary phase while the lower as mobile phase,the flow rate was 2 mL/min,the revolution speed was 850rpm,and the emperature was 25 ℃.The ethyl acetate extracts of Juncus effusus was purified by HSCCC twice.The purity of effusol,dehydroeffusol,juncusol,dehydrojuncusol was 99.3%,98.5%,98.5%,97.4%. Related Articles | Metrics | Comments（0）