To study the chemical constituents from the aerial parts of Blumea balsamifera DC. and their antioxidant and tyrosinase inhibitory activities. Eighteen compounds were isolated and identified from the aerial part of Blumea balsamifera DC. with various chromatographic techniques and spectroscopy methods,which were identified as 7,3′,4′-trimethylquercetin (1),4′,5-dihydroxy-3′,7-dimethoxyflavone (2),luteolin-7-methyl ether (3),rhamnetin (4),(2R,3R)-(+)-3,3′,5,7-tetrahydroxy-4′-methoxydihydroflavone (5),(2R,3R)-(+)-7′-O-methyldihydroquercetin (6),quercetin (7),luteolin (8),eriodictyol (9),isohemiphloin (10),isoquercetin (11),3-methoxyquercetin (12),ursolic acid (13),ursolic acid lactone (14),peroxyergosterol (15),2-hydroxy-4,6-dimethoxyacetophenone (16),2,4-dihydroxy-6-methoxyacetophenone (17),and methyl 3,4-dihydroxyphenyl lactate (18). Among them,compounds 10,14 and 18 were isolated from genus Blumea for the first time,and compound 13 was isolated from B. balsamifera for the first time. The antioxidant and anti-tyrosinase activities of these compounds were screened by using the DPPH free radical method and colorimetric method with L-tyrosine as substrat. The results showed that compounds 3-5,7-12 and 18 have strong antioxidant activities,and compounds 7,13,14,16,17 possessed certain tyrosinase inhibitory effects.