The secondary metabolites of a deep-sea-derived fungus Neoroussoella sp. FS526 and their biological activities were investigated.The solid fermentation extraction of strain FS526 was isolated and purified by silica gel column,Sephadex LH-20 gel column,and high performance liquid chromatography,which led to the isolation of fifteen known compounds.The chemical structures of the isolates were identified by spectrum analysis as amestolkolide B (1),thailandolide B (2),aspergillumarins A-B (3 and 4),de-O-methyldiaporthin (5),4-(2-acetoxyethyl)phenol (6),5,6,8-trihydroxy-4-(1′-hydroxyethyl)isocoumarin (7),sescandelin (8),penicifuran A (9),7-hydroxy-5-methoxy-4,6-dimethylphthalidc (10),pestalotiolactone A (11),2′,4′-dihydroxy-6′-methoxy-3′-methylacetophenone (12),1-(2,4-dihydroxy-6-methoxy-3-methylphenyl)ethanone (13),2,5-dimethylresorcinol (14),and 3,5-dihydroxytoluene (15).All the isolated compounds were firstly reported from the genus Neoroussoella.Compound 1 showed weak cytotoxic activity with the IC₅₀ values of 41.44-77.89 μM,and compound 7 showed strong antioxidant activity with the IC₅₀ value of 13.93 μM.