An endophytic fungus strain L99 from Dongxiang wild rice (Oryza rufipogon Griff.) showed strong inhibitory activity against pathogenic bacteria and fungus.By using morphological characteristics and internal transcribed spacer (ITS) sequence analysis,strain L99 was identified as Sarocladium oryzae.Ten kinds of secondary compounds were separated and purified from fermentation broth of strain L99.According to mass spectrometry,1H NMR and 13C NMR analyses,they were identified as deoxythymidine (1),2′-O-methyladenosine(2) ,2′-deoxyuridine (3),uridine (4),adenosine (5),(E)-9-methyloctadec-9-enoic acid (6),ergosterol peroxide (7),fumonic acid (8),(9Z)-glycerin-1-monooleate (9),and dibutyl phthalate (10).Except for compound 8,the other 9 compounds were isolated from S. oryzae for the first time.The antimicrobial activities were screened,compounds 6,8 and 10 showed certain inhibitory activity against Escherichia coli,Staphylococcus aureus and Xanthomonas oryzae pv. oryzicola,while compound 6 and 9 showed inhibitory activity against Rhizoctonia solani.