Nine compounds were isolated and identified from the fermentation of Colletotrichum sp.B-152,which derived from Dracaena cochinchinensis, by chromatographic and spectroscopic analysis.They are 2-hydroxy-4-methyl-1-phenyl-3-pentanone (1),4-methyl-1-phenyl-2,3-pentanediol (2),thymidine (3),adenosine (4),(22E,24R)-ergosta-7,22-diene-3β,5α,6β-triol（5）,(4E,8E)-N-D-2′-hydroxypalmitoyl-1-O-β-D-glycopyranosyl-9-methyl-4,8-sphingadienine（6）,tuberosider（7）, (22E,24R)-5α,8α-epidioxyergosta-6,22-dien-3β-ol（8）,daturic acid (9).Among which,2-hydroxy-4-methyl-1-phenyl-3-pentanone (1) was reported as natural product for the first time,compounds 2-4,6,7 and 9 were isolated from genus Colletotrichum for the first time.The antimicrobial activities of isolated compounds were determined by paper diffusion method.These 9 compounds showed inhibition abilities against more than one pathogens,compounds 7 and 8 showed broad-spectrum antimicrobial activities.