Antioxidant Activity of Water-soluble Polysaccharides Isolated from Agaricus bisporus

doi:A

Abstract

Abstract: The antioxidant effects of Water Soluble Polysaccharides extracted from Portabella Mushroom Agaricus bisporus (WPPA) were studied by in vitro four different experiments: measuring reducing power, eliminating superoxide anion free radical experiment, eliminating hydroxyl free radical experiment, inhibiting mice erythrocyte hemolysis (MEH) and lipid peroxidation MDA induced by H2O2 experiment. The reducing power of WPPA was excellent. The scavenging abilities of superoxide radical and hydroxyl free radical were notable with IC50 value of 527 and 310 μg/mL, respectively. In addition, WPPA exhibited significant inhibitory actions on MEH and formation of MDA mediated by H2O2 with IC50 value of 700 and 541 μg/mL, respectively. The data obtained in the models in vitro clearly demonstrated the antioxidant potency of the WPPA.

Key words: Agaricus bisporus, polysaccharides, antioxidant activity, erythrocyte hemolysis

CLC Number:

LI Fang-liang, WANG Rui, LIU Ying, GAO Yang, ZHAO Li-dong, ZHANG Feng-ru, HAN Xi-ying. Antioxidant Activity of Water-soluble Polysaccharides Isolated from Agaricus bisporus[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2012, 23(3): 374-377.

References

1 Sun C,Wang JW,Fang L,et al.Free radical scavenging and antioxidant activities of EPS2,an exopolysaccharide produced by a marine filamentous fungus Keissleriella sp.YS 4108.Life Sci,2004,75:1063-1073.
2 Zhao X,Xue CH,Li ZJ,et al.Antioxidant and hepatoprotective activities of low molecular weight sulfated polysaccharide from Laminaria japonica.J Appl Phycol,2004,16:111-115.
3 Ku?ar A,Zupan?i? A,?entjurc M,et al.Free radical scavenging activities of yellow gentian (Gentiana lutea L.) measured by electron spin resonance.Hum Exp Toxicol,2006,25:599-604.
4 Li XL,Zhou AG.Evaluation of the antioxidant effects of polysaccharides extracted from Lycium barbarum.Med Chem Res,2007,15:471-482.
5 Yang XB,Gao XD,Han F,et al.Sulfation of a polysaccharide produced by a marine filamentous fungus Phoma herbarum YS4108 alters its antioxidant properties in vitro.BBA-General Subjects,2005,1725:120-127.
6 Hu HH,Zhang ZY,Lei ZF,et al.Comparative study of antioxidant activity and antiproliferative effect of hot water and ethanol extracts from the mushroom Inonotus obliquus.J Biosci Bioeng,2009,107:42-48.
7 Wang J,Zhang QB,Zhang ZS,et al.Antioxidant activity of sulfated polysaccharide fractions extracted from Laminaria japonica.Int J Biol Macromol,2008,142:127-132.
8 Wang HX,Ng TB.A novel ribonuclease from fresh fruiting bodies of the portabella mushroom Agaricus bisporus.Biochem Cell Biol,2006,84:178-183.
9 Fan Y,Flurkey WH.Purification and characterization of tyrosinase from gill tissue of Portabella mushrooms.Phytochemistry,2004,65:671-678.
10 Kent D,Sheridan CM,Tomkinson HA,et al.Edible mushroom (Agaricus bisporus) lectin inhibits human retinal pigment epithelial cell proliferation in vitro.Wound Repair Regen,2003,11:285-291.
11 Staub AM.Removal of Proteins:Sevag.Methods in Carbohydrate Chemistry,1965,5:5-6.
12 Zhang WJ(张维杰).The Biotechnique of Glycoconjugates (糖复合物生化研究技术).Hangzhou:Press of Zhejiang University,2003,11-12.
13 Marklund S,Marklund G.Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase.Eur J Biochem,1974,47:469-474.
14 Smirnoff N,Cumbes QJ.Hydroxyl radical scavenging activity of compatible solutes.Phytochemistry,1989,28:1057-1060.
15 Record IR,MacQueen SE,Dreosti IE.Zinc,iron,vitamin E,and erythrocyte stability in the rat.Biol Trace Elem Res,1990,23:89-96.

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