Abstract: Laccase is a kind of multifunctional enzyme which has extensive applied foreground, but output-lacking becomes a major problem for its use. Basidiomycota the major source for laccase secretion. This paper studied the property of extracellular laccase in cultures of Grifola frondosa which belonged to Basidiomycota fungi. The optimal temperature and pH value for laccase activity were 65 ℃ and pH 2.2, respectively. Enzyme activity was also affected by buffer composition. G. frondosa laccase was relatively heat stable. Halide ions could strongly inhibit laccase activity.

Key words: Grifola frondosa, extracellular laccase, pH value, temperature, anions, cations