Abstract: The total content of resveratrol and polydatin was used as the investigation index.The efficiency of ultrasonic extraction,heating reflux extraction and accelerated solvent extraction (ASE) for the extraction of resveratrol and polydatin in cultivated Polygonum multiflorum from Qinghai was compared.The extraction conditions of ASE were optimized through single-factor test and response surface methodology (RSM).To obtain the maximum of extraction yield of resveratrol and polydatin, three main factors including ethanol concentration,extraction temperature and extraction time were selected and further optimized using RSM on the basis of single factor experimental results.As a result,ASE was markedly superior to the other two methods.The optimal conditions of ASE were as follows:51% of aqueous ethanol,extraction temperature of 96 ℃,and extraction time of 16 min.ASE was simple,rapid and efficient,and was appropriate for the extraction of resveratrol and polydatin in P.multiflorum.

Key words: cultivated Polygonum multiflorum, resveratrol, polydatin, accelerated solvent extraction, response surface methodology