Abstract: To establish an UHPLC method for simultaneous determination of six compounds,namely chlorogenic acid,rutin,luteoloside,3,5-O-dicaffeoylquinic acid, 3,4-O-dicaffeoylquinic acid and 4,5-O-dicaffeoylquinic acid in Lonicera japonica Thunb from different areas.The chromatographic separation was performed on an Agilent ZORBAX RH C₁₈ (50 mm × 2.1 mm,1.8 μm) column with gradient elution.The flow rate was 0.2 mL/min.The column temperature was 30 ^oC.The detection wavelength was 350 nm.The recoveries of rutin,luteoloside and four kinds of organic acids were 96.88%-99.16%,and the relative standard deviations were 0.23%-1.06%.The developed UHPLC method was reproducible,accurate and efficient.It can be used for the simultaneous detection and determination of chlorogenic acid,rutin,luteoloside,3,5-O-dicaffeoylquinic acid,3,4-O-dicaffeoylquinic acid,4,5-O-dicaffeoylquinic acid in L.japonica.

Key words: Lonicera japonica Thunb, UHPLC, chlorogenic acid, rutin, luteoloside, 3,5-O-dicaffeoylquinic acid, 3,4-O-dicaffeoylquinic acid, 4,5-O-dicaffeoylquinic acid