Abstract: The contents of two lignans,namely podophyllotoxin and 4′-demethylpodophyllotoxin in cultivated and wild Sinopodophyllum hexandrum (Royle) Ying from Qinghai were extracted by ultrasonicaction and analyzed by HPLC.The results showed that the contents of podophyllotoxin distributed in cultivated and wildS.hexandrum was as follows:rhizome> petiole > leaf.The contents of podophyllotoxin in petioles and leaves of cultivated plants were higher than these of the wild plants;The content of 4′-demethylpodophyllotoxin distributed in cultivated plants was as follows:petiole > rhizome,no detection of 4′-demethypodophyllotoxin in leaves of cultivated plants;The content of 4′-demethylpodophyllotoxin distributed in wild plants was as follows:lateral root > petiole> rhizome> leaf,no detection of 4′-demethypodophyllotoxin in fruit.The content of 4′-demethylpodophyllotoxin in petioles of cultivated plants was higher than that of the wild plant.

Key words: cultivated, Sinopodophyllum hexandrum (Royle) Ying, HPLC, podophyllotoxin, 4&prime, -demethylpodophyllotoxin