Abstract: Ten chemical constituents were isolated and purified from the solid culture of endophytic fungal strain Botryosphaeria rhodina A13 isolated from Aquilaria sinensis by silica gel column chromatography,reverse silica gel column chromatography,Sephadex LH20 column chromatography,HPLC,preparative TLC and recrystallization. On the basis of spectral data and physicochemical properties,their structures were identified as 3-oxo-Δ-4,5-sitosterone (1), (24R)-3β-hydroxyethylcholest-5-en-7-one (2), stigmast-4-ene-3,6-dione (3), (22E,24S)-5α,6α-epoxy-24-methylcholesta-8(14),22-diene-3β,7α-diol (4),genkwanin (5),benzoic acid (6),4-methoxybenzoic acid (7),pyrimidine-2,4(1H,3H)-dione (8),2-methoxyhydroquinone (9),(-)-gynuraone (10).All the compounds were isolated from the genus Botryosphaeria for the first time.Compound 4 showed cytotoxic activities against MCF-7 and NCI-H460 cell lines.

Key words: Botryosphaeria rhodina, endophytic fungus, secondary metabolite, Aquilaria sinensis ( Lour.) Gilg, cytotoxicity