Abstract: The conditions for complex enzymatic extraction of lycorine from Lycoris aurea were optimized by L₉(3⁴) orthogonal array design.Lycorine was further separated using cation exchange resin.Celluase and pectinase solution was used as the extraction solvent.The effects of pH value of enzyme,amount of complex enzyme,dissociation time and enzymatic hydrolysis temperature on the extraction results were investigated.The optimal conditions were obtained as follows:ratio of solid to liquid was 1:10,pH value was 4.5,amount of complex enzymes was 4%,enzymatic hydrolysis temperature was 50 ℃ and reaction time was 2.0 h.Under these conditions,the extraction yield of lycorine was 0.1750%.In addition,D-001 cation exchange resin was selected for the separation of lycorine.The separation conditions were obtained as follows:adsorption flow rate was 3 BV/h with reagent of pH 2 and the desorption flow rate was 3 BV/h with 70% ethanol solution containing 1.5 mol/L ammonia.After separation,the content of lycorine was increased to 15.28%.The results can provide a reference for industrial production of lycorine.

Key words: Lycoris aurea, lycorine, complex-enzymatic extraction, cation exchange resin, separation