天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (1): 84-88.doi: 10.16333/j.1001-6880.2015.01.018

• 研究简报 • 上一篇    下一篇

近红外漫反射技术测定烤烟烟叶中的茄尼醇

付秋娟1,杜咏梅1*,张怀宝1,侯小东1,李燕华2,李丹丹1   

  1. 1 中国农业科学院烟草研究所,青岛 266101;2 山东京鲁烟叶复烤有限公司,潍坊 262200
  • 出版日期:2015-01-31 发布日期:2015-02-12

Determination of Solanesol Content of Flue-Cured Tobacco Leaves by Near-Infrared Diffuse Reflectance Technology

FU Qiu-juan1, DU Yong-mei1*, ZHANG Huai-bao1, HOU Xiao-dong1, LI Yan-hua2, LI Dan-dan1   

  1. 1 Tobacco Research Institute of CAAS,Qingdao 266101,China; 2 Shandong Jinglu tobacco redrying Co.Ltd,Weifang 262200,China
  • Online:2015-01-31 Published:2015-02-12

摘要: 为研究烟草茄尼醇的近红外光谱快速检测技术,采用偏最小二乘法(PLS),选择合适的预处理方法,建立了烤烟烟叶中茄尼醇的近红外光谱漫反射定量校正模型,模型交叉验证均方差RMSECV为0.108,外部验证的预测均方差RMSEP为0.0822,平均相对偏差为5.3%,说明模型可以对茄尼醇进行快速准确地预测。并通过有标转移法成功地将模型转移到其它仪器上,实现了烟草茄尼醇在不同实验室的快速检测。

关键词: 近红外漫反射, 烤烟, 茄尼醇, 模型

Abstract: In order to determinate the content of solanesol in flue-cured tobacco leaves by near-infrared diffuse reflectance technology,the near-infrared quantitative calibration model was established using the method of partial least squares (PLS) based on optimizing the spectral data pretreatment method.The crossvalidation RMSECV of calibration model was 0.108,the external-validation RMSEP of calibration model was 0.0822,the average relative deviation was 5.3%, which indicated solanesol content can be predicted quickly and accurately according to the established model.The near-infrared quantitative calibration model was also transferred to other machines by marking transfer method.This achieved rapid detection of solanesol content in different laboratories.

Key words: near-infrared diffuse reflectance, flue-cured tobacco, solanesol, calibration model

中图分类号: 

O657.33 TS41+1