天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (2): 232-235.doi: 10.16333/j.1001-6880.2015.02.007

• 研究论文 • 上一篇    下一篇

深红酵母JLC固态发酵废弃烟梗制备类胡萝卜素研究

郭大城1,张可可2,董贵彬2,张旭萍2,刘慧2,朱大恒2,席宇2*   

  1. 1 河南省疾病预防与控制中心,郑州 450016;2 郑州大学生命科学学院,郑州 450001
  • 出版日期:2015-02-15 发布日期:2015-02-15

Carotenoids Production by Rhodotorula rubra JLC under Solid State Fermentation of Waste Tobacco Stems

GUO Da-cheng1, ZHANG Ke-ke2, DONG Gui-bin2, ZHANG Xu-ping2, LIU Hui2, ZHU Da-heng2, XI Yu2*   

  1. 1 Henan Provincial Center for Disease Control and Prevention,Zhengzhou 450016,China; 2 School of Life Science,Zhengzhou University,Zhengzhou 450001,China
  • Online:2015-02-15 Published:2015-02-15

摘要: 烟梗是卷烟工业的一种典型固态废弃物,为资源化利用废弃烟梗(waste tobacco stem,WTS),采用Plackett-Burman(PB)设计对影响一株深红酵母(Rhodotorula rubra)JLC固态发酵WTS生产类胡萝卜素的相关因素进行了评价。PB设计与统计学分析表明:在选择的10个相关因素中,装载量、培养时间和酵母粉添加量是影响类胡萝卜素产量的3个关键因素。在PB实验条件下,深红酵母JLC发酵产类胡萝卜素的最高量可达21.54 μg/g。

关键词: 烟梗, 固态发酵, 类胡萝卜素, Plackett-Burman设计, 深红酵母

Abstract: Tobacco stem is a typical solid waste in the tobacco industry,to explore technologies for the resource utilization of waste tobacco stems (WTS),Plackett-Burman (PB) design was employed to evaluate some variables which were relevant to carotenoids production of Rhodotorula rubra JLC under solid state fermentation of WTS.Among ten selected variables,loading capacity,culturing time and yeast powder,as three key factors,were found to significantly influence carotenoids production via PB design and the following statistic analysis.Under PB design conditions,the maximum production of carotenoids reached 21.54 μg/g.

Key words: tobacco stem;solid state fermentation;carotenoids, Plackett-Burman design;Rhodotorula rubra

中图分类号: 

Q932