天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (3): 455-458.doi: 10.16333/j.1001-6880.2015.03.017

• 研究简报 • 上一篇    下一篇

杭黄菊提取物的体外抗菌活性研究

宋雷1*,绽春蕊1,杨世英1,马伟林1,马贤1,柳军席2   

  1. 1 西北民族大学医学院,兰州 730030;2 中国科学院兰州化学物理研究所甘肃省天然药物重点实验室,兰州 730000
  • 出版日期:2015-03-30 发布日期:2015-04-07

Anti-bacterial Activity of Chrysanthemum morifolium Ramat

SONG Lei1*,ZHAN Chun-rui1,YANG Shi-ying1,MA Wei-lin1,MA Xian1,LIU Jun-xi2   

  1. 1 Medical College of Northwest University for Nationalities,Lanzhou 730030,China;2 Key Laboratory for Natural  Medicine of Gansu Province,Institute of Chemical Physics,Chinese Academy of Sciences,Lanzhou 730000,China
  • Online:2015-03-30 Published:2015-04-07

摘要: 本文研究杭黄菊花水提物和醇提物对常见的人体肠道致病菌的抑菌活性。采用超声波提取法获得杭黄菊提取物,用琼脂扩散法测试该粗提物对受试菌的抑菌作用,并用倍比稀释法确定最小抑菌浓度。实验结果表明杭黄菊花水提物和醇提物对常见的7种肠道致病菌具有较强的抑制作用,尤其对变形杆菌、痢疾杆菌和伤寒杆菌的抑制作用较强。采用相同提取方法的高浓度提取物比低浓度提取物具有更显著(P<0.01)的抑菌作用,而相同浓度的水提物比醇提物抑菌效果更为明显(P<0.01),水提物对相同细菌的最低抑菌浓度(MIC)低于醇提物。杭黄菊提取物对肠道致病菌有明显的抑制作用,可被开发用于抗菌新药研制。

关键词: 杭黄菊, 提取物, 肠道菌群, 抑菌作用

Abstract: The anti-bacterial effects of crude extracts of flower of Chrysanthemum morifolium Ramat (CMR) on common human intestinal bacteria were investigated in this study.Anti-bacterial activities of ultrasonic extracts of CMR flower were tested by agar diffusion method,and minimal inhibitory concentrations (MIC) were determined by multi-proportion dilution method.The results showed that both aqueous extract and alcohol extract showed high inhibitory effect on 7 common human intestinal bacteria,especially on Proteus vulgaris, Shigella dysenteriae and Salmonella typhi. The anti-bacterial effects of aqueous and alcohol extracts with higher concentration were significantly obvious (P<0.01) than that of lower concentration,and aqueous extract showed prominent (P<0.01) anti-bacterial effects than alcohol extract at the same concentration.Furthermore,the MIC of aqueous extract lower than that of alcohol extract for the same bacterium.

Key words: Yellow chrysanthemum, extracts, intestinal bacteria, bacteriostasis

中图分类号: 

R284.2 R285.5 TS201.3