天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (3): 496-501.doi: 10.16333/j.1001-6880.2015.03.026

• 开发研究 • 上一篇    下一篇

酶水解制备山药皮可溶性膳食纤维及性能测定

曲玲玉1,李大为1,张鹏1,朱运平1,2,3,李秀婷1,2,3,滕超1,2,3*   

  1. 1 北京工商大学食品学院;2 京工商大学食品质量与安全北京实验室;3 北京工商大学北京市食品添加剂工程技术研究中心,北京100048
  • 出版日期:2015-03-30 发布日期:2015-04-07

Preparation and Analysis of Soluble Dietary Fiber from the Peel of Rhizoma Dioscoreae by Enzymatic Method

QU Ling-yu1,LI Da-wei1,ZHANG Peng1,ZHU Yun-ping1,2,3,LI Xiu-ting1,2,3,TENG Chao1,2,3*   

  1. 1 Beijing Laboratory of Food Quality and Safety,Beijing Technology & Business University;2 Food quality and safety Lab,Beijing Technology & Business University; 3 Beijing Engineering and Technology Research Center of  Food Additives,Beijing Technology & Business University,Beijing 100048,China
  • Online:2015-03-30 Published:2015-04-07

摘要: 本研究以山药皮为原料研究纤维素酶法制备可溶性膳食纤维工艺条件,研究考察了料液比、加酶量、提取时间、提取温度、醇沉时间等因素,确定最佳工艺条件为:料液比1:30、酶添加量4 U/mL、提取时间2 h、提取温度50 ℃,醇沉时间4 h。通过响应面法对工艺条件进一步优化,确定料液比1:35,酶添加量5 U/mL,温度48 ℃时,SDF制备率最高达22.87%,较初始SDF含量7.96%提高近3倍。同时对制备的SDF性能进行测定,其持水性可达到10.74 g/g,溶胀力为6.45 mL/g,具备了一定的工业应用潜力。

关键词: 山药皮, 水溶性膳食纤维, 酶水解, 响应面分析

Abstract: Preparation of soluble dietary fiber (SDF) from the peel of Rhizoma Dioscoreae by cellulase hydrolysis was studied.In order to optimize conditions,conventional single factor method was designed and the outcomes were as follows:solid/liquid ratio of 1:30,the dosage of cellulose of 4 U/mL,extraction time of 2 h,extraction temperature of 50 oC and alcohol precipitation time of 4 h,respectively.For further enhance the amount of soluble fiber,response surface analysis method was carried out.The conditions were further optimized as:solid/liquid ratio of 1:35,the dosage of cellulase of 5 U/mL,extraction temperature of 48 oC.Under these conditions,the yield of SDF was 22.87%,which was 3 times of the initial yield of 7.96%.In addition,the properties of the resulting SDF were also investigated.The water holding capacity and swelling capacity were 10.74 g/g and 6.45 mL/g,respectively.

Key words: peel of Rhizoma Dioscoreae, soluble dietary fiber, enzymatic hydrolysis, response surface methodology

中图分类号: 

TS201.2