天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (3): 534-539.doi: 10.16333/j.1001-6880.2015.03.033

• 开发研究 • 上一篇    下一篇

大孔吸附树脂纯化绿茄叶黄酮的工艺研究

莫天录1，薛林贵1*，高慧2，李彩霞3，李旭东1，李文伟1，李国强1，杨蕊琪1   

  1. 1 兰州交通大学化学与生物工程学院，兰州 730070；2 兰州大学 生命科学学院，兰州 730020；3 河西学院农业与生物技术学院，张掖 734000
  • 出版日期:2015-03-30 发布日期:2015-04-07

Purification of Flavonoids from Green Eggplant Leaves Using Macroporous Resin

MO Tian-lu1,XUE Lin-gui1*,GAO Hui2,LI Cai-xia3,LI Xu-dong1,LI Wen-wei1,LI Guo-qiang1,YANG Rui-qi1   

  1. 1 School of Chemical and Biological Engineering, Lanzhou Jiaotong University, Lanzhou,730070,China; 2 School of Life Science, Lanzhou University, Lanzhou 730020，China; 3School of Agriculture and Biotechnology, Hexi University,Zhangye 734000,China
  • Online:2015-03-30 Published:2015-04-07

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摘要： 研究大孔吸附树脂纯化绿茄叶黄酮粗提取物的最佳工艺。通过比较10种大孔吸附树脂纯化黄酮粗提取物的吸附及解吸性能，筛选出纯化树脂XDA-1，并考察XDA-1树脂对黄酮粗提取物的静态、动态吸附与解吸的性能。结果表明，XDA-1树脂对黄酮粗提取物纯化的最佳工艺参数：吸附平衡时间8 h，吸附浓度2.00 mg/mL，pH值3.0，温度25 ℃，上样流速2 BV/h；解吸平衡时间2 h，解吸剂为pH值为3.0的体积分数80%的乙醇溶液，解吸流速3 BV/h，纯化倍数2.37。该研究证实大孔吸附树脂纯化绿茄叶黄酮的方法简单可行，为绿茄叶黄酮的分离纯化提供了实验依据。

关键词: 大孔吸附树脂, 绿茄叶, 黄酮, 纯化

Abstract: The optimal conditions for purifying flavonoids from green eggplant leaves using macroporous resin were studied.The absorption and desorption properties of 10 different macroporous resins for purifying flavonoids from green eggplant leaves were compared.The results showed that XDA-1 had the best adsorption and desorption performance.Furthermore,the static and dynamic absorption and desorption properties of XDA-1 on flavonoids were investigated.The optimal conditions for the separation and purification of flavonoids from green eggplant leaves were listed as follows:equilibrium adsorption time,8 hrs;concentration,2.00 mg/mL;pH,3;temperature,25 ℃;velocity of sample solution,3 BV/hrs;equilibrium desorption time,2 hrs;elution velocity,3 BV/hrs;and mobile phase solvent,using 80% aqueous ethanol (pH=3).The flavonoid content in the purified product was 2.37 times higher than that in the raw material.In conclusion,our study provided a simple and feasible purification method for purifying flavonoids from the leaves of green eggplant.

Key words: macroporous resin, green eggplant leaves, flavonoids, purification

中图分类号: 

Q283.6

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