天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (6): 1011-1015.doi: 10.16333/j.1001-6880.2015.06.013

• 研究简报 • 上一篇    下一篇

刺玫果果肉石油醚层化学成分及胰脂酶和α-糖苷酶抑制活性研究

李小第1,姜会敏2,霍雅玉2,高阳2,鲍和1,张海龙2*   

  1. 1 西安交通大学医学部第二附属医院药剂科,西安 710004;2 西安交通大学医学部药学院,西安 710061
  • 出版日期:2015-06-30 发布日期:2015-07-10

Chemical Constituents of Petroleum Ether Fraction of the Pericarps of Rosa davurica Pall.and Their Inhibitory Activity on Lipase and α-Glucosidase

LI Xiao-di1, JIANG Hui-min2, HUO Ya-yu2, GAO Yang2, BAO He1, ZHANG Hai-long2*   

  1. 1 Department of Pharmacy,the Second Affiliated Hospital,Xian Jiaotong University,Xian 710004,China; 2 School of Pharmacy,Health Science Center,Xi'an Jiaotong University,Xi'an 710061,China
  • Online:2015-06-30 Published:2015-07-10

摘要: 采用硅胶柱色谱、葡聚糖凝胶柱色谱等方法进行分离纯化,通过波谱数据进行结构鉴定,对石油醚层通过荧光检测法测定胰脂肪酶抑制活性,通过96微孔板法测定不同来源α葡萄糖苷酶的抑制活性。从刺玫果果肉石油醚层分离得到12个化合物,分别鉴定为二十九烷(1)、α-生育酚(2)、邻苯二甲酸二乙酯(3)、邻苯二甲酸二丁酯(4)、β-谷甾醇(5)、α-香树脂醇(6)、乌苏醇(7)、白桦脂醇(8)、白桦脂酸(9)、19α-羟基乌苏酸(10)、胡萝卜苷(11)和麦芽糖(12)。化合物1~3、6~7和12首次从该植物中分离得到,石油醚层对胰脂肪酶和酵母菌来源的α-葡萄糖苷酶具有一定的抑制活性。

关键词: 刺玫果, 果肉, 化学成分, 胰脂肪酶, &alpha, -葡萄糖苷酶

Abstract: Twelve compounds were isolated and purified from petroleum ether fraction of the pericarps of Rosa davurica Pall.by silica gel and Sephadex LH-20 column chromatography. Their structures were elucidated on the basis of physicochemical properties and spectroscopic data as nonacosane (1),α-tocopherol (2),diethyl phthalate (3),dibutyl phthalate (4), β-sitosterol (5), α-amyrin(6),uvaol (7),botulin (8),betulic acid (9),19-α-hydroxyursolic acid (10),daucosterol (11) and maltose (12).Compounds 1-3, 6-7 and 12 were isolated from R.davurica for the first time.Petroleum ether fraction showed some inhibitory activity on the pancreatic lipase by fluorescent method and on α-glucosidase from the yeast by 96-microplate based method.

Key words: Rosa davurica Pall., pericarps, chemical constituents, pancreatic lipase, &alpha, -glucosidase

中图分类号: 

R284.2