天然产物研究与开发 ›› 2016, Vol. 28 ›› Issue (1): 96-101.doi: 10.16333/j.1001-6880.2016.1.017

• 研究简报 • 上一篇    下一篇

超声辅助提取四齿四棱草中总黄酮及其抗氧化活性

陈飞1，何先元1,2*，周卯勤2，程鹏3，欧阳凯1，黄英如1,3   

  1. 1 重庆医科大学中医药学院，重庆 401331；2 重庆市药物种植研究所，重庆 408435；3 重庆医科大学实验管理中心，重庆 401331
  • 出版日期:2016-01-29 发布日期:2016-02-01

Ultrasonic-assisted Extraction and Antioxidant Effects of Total Flavonoids from Schnabelia tetradonta

CHEN Fei1,HE Xian-yuan1,2*,ZHOU Mao-qin2,CHENG Peng3,OU YANG Kai1, HUANG Ying-ru1,3   

  1. 1 College of Traditional Chinese Medicine,Chongqing Medical University,Chongqing 401331,China; 2 Chongqing Institute of Medicinal Plant Cultivation,Chongqing 408435,China; 3 Experimental management center of Chongqing Medical University,Chongqing 401331,China
  • Online:2016-01-29 Published:2016-02-01

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摘要： 本文采用正交实验优选超声辅助提取四齿四棱草中总黄酮的最佳工艺，测定其含量，并以Vc为对照，采用DPPH法、ABTS法和FRAP法对四齿四棱草总黄酮抗氧化活性进行综合评价。通过单因素实验和正交实验，研究乙醇浓度、超声时间、料液比、提取温度对四齿四棱草中总黄酮提取率的影响，优选出超声辅助提取四齿四棱草总黄酮的最佳工艺：乙醇浓度为70%，料液比为1:30 g/mL，50 ℃超声提取两次，每次30 min，四齿四棱草总黄酮提取率为4.78%。四齿四棱草总黄酮对DPPH自由基（IC50=42.91 μg/mL，最大清除率为88.57%）、ABTS自由基（IC50=44.61 μg/mL，最大清除率为92.86%）的清除能力较好，对Fe3+的还原能力（AEAC＝5380 μmol/g）亦较强，是一种天然的抗氧化剂。

关键词: 四齿四棱草, 超声辅助提取, 总黄酮, DPPH, ABTS, FRAP

Abstract: The objectives of this study were to optimize the ultrasonic-assisted extraction process of total flavonoids from Schnabelia tetradonta with orthogonal design and to determinate its antioxidant activity. The antioxidant activity of S. tetradonta was evaluated by three methods:DPPH radical scavenging,ABTS radical scavenging and FRAP assay,with Vc as the control. The single factor test and orthogonal design were used to select the optimal extraction condition with ultrasonication by studying the effect of ethanol concentration,ultrasonic time,solid-liquid ratio and extraction temperature on the extraction yield of flavonoids. The experimental results revealed that:the optimal extraction conditions were:ethanol concentration of 70%,solid-liquid ratio of 1:30 g/mL,and extracting 2 times at 50 ℃,30 min each time. Under these conditions,the extraction yield of total flavonoids reached up to 4.78%. In addition,total flavonoids of S. tetradonta showed strong DPPH radical scavenging activity (IC50=42.91 μg/mL,the maximum scavenging rate was 88.57%),ABTS radical scavenging activity (IC50=44.61 μg/mL,the maximum scavenging rate was 92.86%) and ferric reducing antioxidant power (AEAC=5380 μmol/g). It is an effective and natural antioxidant.

Key words: Schnabelia tetradonta, ultrasonic-assisted extraction, total flavonoids, DPPH, ABTS, FRAP

中图分类号: 

R284.2

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