天然产物研究与开发 ›› 2016, Vol. 28 ›› Issue (1): 131-135.doi: 10.16333/j.1001-6880.2016.1.023

• 开发研究 • 上一篇    下一篇

3,5,2′,4′-四羟基查尔酮对小鼠尿酸及尿酸合成相关酶基因的影响

刘恺1,2，林华1，高丽辉1，刘旭1，李玲1，牛艳芬1*   

  1. 1 昆明医科大学生物医学工程研究中心，昆明 650500；2 衡阳市中心医院药学部临床药学室，衡阳 421001
  • 出版日期:2016-01-29 发布日期:2016-02-01

Effects of 3,5,2′,4′-Tetrahydroxychalcone on Serum Uric Acid Levels and Some Key Enzymes Involved in Uric Acid  Production in Oxonate-induced Hyperuricemic Mice

LIU Kai1,2,LIN Hua1,GAO Li-hui1,LIU Xu1,LI Ling1,NIU Yan-fen1*   

  1. 1 Biomedical Engineering Research Center,Kunming Medical University,Kunming 650500,China; 2 Central Hospital of Hengyang,Hengyang 421001,China
  • Online:2016-01-29 Published:2016-02-01

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摘要： 用氧嗪酸钾诱导高尿酸血症动物模型，对化合物3,5,2′,4′-四羟基查尔酮（P40）的降尿酸作用及尿酸合成相关酶基因进行研究。用磷钨酸法测定小鼠血清尿酸水平，用RT-PCR测定脑组织中次黄嘌呤鸟嘌呤磷酸核糖转移酶（HGPRT）、肝脏中的磷酸核糖焦磷酸合成酶（PRPS）和磷酸核糖焦磷酸酰胺转移酶（PRPPAT）mRNA的表达水平。结果表明：灌胃给予高尿酸血症小鼠 P40 2、4、8 mg/kg和阳性对照药别嘌醇1 mg/kg，共给药5次，每天2次，均显著降低血清尿酸水平（P＜0.05，0.01），具有显著的降尿酸作用；但对HGPRT、PRPS、PRPPAT的mRNA表达水平无明显影响。

关键词: 3, 5, 2&prime, 4&prime, -四羟基查尔酮, 高尿酸血症, 尿酸, 次黄嘌呤鸟嘌呤磷酸核糖转移酶, 磷酸核糖焦磷酸合成酶, 磷酸核糖焦磷酸酰胺转移酶

Abstract: The aim of this study was to investigate the effects of 3,5,2′,4′-tetrahydroxych alcone (P40) on serum uric acid level,the mRNA expression level of hypoxanthineguanine phosphoribosyl transferase (HGPRT) of brain,5’-phosphoribosyl-1’-pyrophosphate synthetase (PRPS) and phosphoribosyl amidotransferase (PRPPAT) of liver in hyperuricemic mice. The hyperuricemic mice were induced by an intraperitoneal injection of uricase inhibitor potassium oxonate. All drugs were given intragastrically twice daily for five doses. Serum uric acid levels were determined at 1 h after the last dosage. The effects of P40 with different doses on HGPRT,PRPS and PRPPAT mRNA expression level were examined by the means of RT-PCR. The results showed serum uric acid levels in the model group were increased obviously (P<0.01). Compared with the model group,serum uric acid levels were decreased significantly in groups received P40 at the doses of 2,4,8 mg/kg (P<0.05 or P<0.01). P40 at doses of 2,4,8 mg/kg had no significant effect on the mRNA expression of HGPRT,PRPS and PRPPAT in hyperuricemic mice (P>0.05). Hence,P40 can reduce the serum uric acid levels in oxonate-induced hyperuricemic mice,but not inhibiting the mRNA expression of HGPRT in brain as well as PRPS and PRPPAT in liver under the experimental condition.

Key words: 3,5,2&prime, ,4&prime, -tetrahydroxychalcone, hyperuricemia, hypoxanthine guanine phosphoribosyl transferase, 5&prime, -phosphoribosyl-1&rsquo, -pyrophosphate synthetase, phosphoribosyl amidotransferase

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R965.1

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