天然产物研究与开发 ›› 2016, Vol. 28 ›› Issue (11): 1771-1776.doi: 10.16333/j.1001-6880.2016.11.015

• 研究简报 • 上一篇    下一篇

三种方法测定鲨鱼硫酸软骨素钠含量的比较

焦广飞*   

  1. 福建海天蓝生物制品有限公司,龙岩 364000
  • 出版日期:2016-11-30 发布日期:2017-03-30

Comparison of Three Methods for Determination of Chondroitin Sulfate Sodium Shark

JIAO Guang-fei*   

  1. Fujian Hai Tian Lan Bio-products Co.,Ltd.Longyan 364000,China
  • Online:2016-11-30 Published:2017-03-30

摘要: 利用酶解高效液相色谱法、高效液相色谱法及氯化十六烷基吡啶电位滴定法对鲨鱼硫酸软骨素钠含量进行检测,并对检测结果进行对比分析,结果显示,酶解高效液相色谱法不适用于鲨鱼硫酸软骨素钠含量的测定;而氯化十六烷基吡啶电位滴定法检测鲨鱼硫酸软骨素钠含量稍高于高效液相色谱法;相比之下,高效液相色谱法较适用于检测鲨鱼硫酸软骨素钠含量。

关键词: 酶解高效液相色谱法, 高效液相色谱法, 氯化十六烷基吡啶电位滴定法, 鲨鱼硫酸软骨素钠

Abstract: The content of chondroitin sulfate sodium shark (CS) was separately evaluated by enzymatic-high performance liquid chromatography (E-HPLC),high performance liquid chromatography (HPLC) and cetylpyridinium chloride (CPC)-titration methods.After comparing and analyzing the detection results,it was found that E-HPLC method was not suitable for determining chondroitin sulfate sodium shark.Moreover,the content determined by CPC-titration method was higher than that of HPLC method.By contrast,HPLC was more suitable for determining of chondroitin sulfate sodium shark.

Key words: enzymatic-high performance liquid chromatography, high performance liquid chromatography, cetylpyridinium chloride-titration, chondroitin sulfate sodium shark

中图分类号: 

R927.2