光裸方格星虫纤溶酶SNFE体外溶栓作用及安全性初评

doi:10.16333/j.1001-6880.2016.11.018

天然产物研究与开发 ›› 2016, Vol. 28 ›› Issue (11): 1789-1792.doi: 10.16333/j.1001-6880.2016.11.018

光裸方格星虫纤溶酶SNFE体外溶栓作用及安全性初评

李映新1，李肖肖2，黄媛恒3，黎钦蓉4，雷丹青5*

1广西医科大学药学院，南宁 530021；2桂东人民医院，梧州 543001；3广西医科大学基础医学院；4广西医科大学全科医学院；5广西蛇毒研究所，南宁 530021

出版日期:2016-11-30 发布日期:2017-03-30

The in vitro Anti-thrombolytic Effect and Bio-security of Fibrinolytic Enzyme SNFE from Sipuculus nudus Linnaeus in Guangxi Coastal Area of China

LI Ying-xin1,LI Xiao-xiao2,HUANG Yuan-heng3,LI Qin-rong4,LEI Dan-qing5*

1 Pharmaceutical school,Guangxi medical University,Nanning 530021,China; 2 Guidong people’s hospital, Wuzhou 543001,China; 3 School of preclinical medicine,Guangxi Medical University;4General medical school  of Guangxi Medical University; 5The Institute of Snake Venom Research of Guangxi Medical University,Nanning 530021,China

Online:2016-11-30 Published:2017-03-30

摘要/Abstract

摘要： 研究光裸方格星虫纤溶酶SNFE体外溶栓作用并初步评价药物的生物安全性。通过标准纤维蛋白平板和加热纤维蛋白平板实验、剩余可凝纤维蛋白原法、体外溶栓实验研究SNFE体外溶栓作用及作用方式；通过皮下出血实验及体外溶血实验初步评估SNFE的生物安全性。结果发现，SNFE在体外不仅具有激酶活性，且能直接溶解纤维蛋白原和纤维蛋白，对血凝块具有良好的溶栓作用，同时无出血活性及溶血作用，具有一定的开发价值。

关键词: 溶栓作用, 纤溶酶, 光裸方格星虫

Abstract: The aim of this study was to investigate the in vitro anti-thrombolytic effect and to preliminary evaluate on the bio-security of the fibrinolytic enzyme SNFE from Sipuculus nudus Linnaeus in Guangxi Coastal Area of China.The in vitro anti-thrombolytic effect and action mode of SNFE were elucidated using the plasminogen free and rich wells fibrin plate assay,detecting the residual of thrombin clottable fibrinogen contents,and thrombolytic experiment.The bio-security was assessed by subcutaneous hemorrhage and hemolysis experiments.Results showed that SNFE not only had the kinase activity,but also directly dissolved fibrin and fibrinogen,it can induce clot lysis in vitro, and had no obvious hemolysis and hemorrhagic effects.

Key words: anti-thrombolytic effect, fibrinolytic enzyme, Sipuculus nudus Linnaeu

中图分类号:

R963

李映新1，李肖肖2，黄媛恒3，黎钦蓉4，雷丹青5*. 光裸方格星虫纤溶酶SNFE体外溶栓作用及安全性初评[J]. 天然产物研究与开发, 2016, 28(11): 1789-1792.

LI Ying-xin1,LI Xiao-xiao2,HUANG Yuan-heng3,LI Qin-rong4,LEI Dan-qing5*. The in vitro Anti-thrombolytic Effect and Bio-security of Fibrinolytic Enzyme SNFE from Sipuculus nudus Linnaeus in Guangxi Coastal Area of China[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2016, 28(11): 1789-1792.

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光裸方格星虫纤溶酶SNFE体外溶栓作用及安全性初评

The in vitro Anti-thrombolytic Effect and Bio-security of Fibrinolytic Enzyme SNFE from Sipuculus nudus Linnaeus in Guangxi Coastal Area of China

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