关键词: 燕麦麸皮球蛋白, 转谷氨酰胺酶, 糖基化, 接枝度, 响应面

Abstract: The glycosylated oat bran globulin with higher grafting degree was produced by the catalysis of transglutaminase(TG).Single factor design and response surface methodology(RSM)were used to optimize the grafting condition.The results indicated that the optimal conditions were ratio of amino sugars and globulin concentration of 0.94:1,TG adding amount of 57.59 U/g,pH 7.65,4 h and 45 ℃.Under these conditions,the grafting degree of glycosylated oat bran globulin was 45.6774%,which was consistent with the predicated value.This modified protein system with 0.379 mol/kg of free amino further confirmed that the transglutaminase-catalyzed oat bran globulin presented better grafting system than the original one.The results of this study provided a potential further application for the oat bran protein in development of new product.

Key words: oat bran globulin, transglutaminase, glycosylation, grafting degree, response surface methodology