超高液相色谱法同时测定北五味子中7种木脂素类化合物

doi:10.16333/j.1001-6880.2017.4.013

天然产物研究与开发 ›› 2017, Vol. 29 ›› Issue (4): 605-609.doi: 10.16333/j.1001-6880.2017.4.013

超高液相色谱法同时测定北五味子中7种木脂素类化合物

白宏博1，冯志强2，史鑫波1，孙晓春1，刘妍如1*，唐志书1*

1陕西中医药大学陕西省中药资源产业化协同创新中心，陕西省中药基础与新药研究实验室，咸阳 712083；2西安万隆制药股份有限公司，西安 710119

出版日期:2017-04-29 发布日期:2017-05-08

Simultaneous Determination of Seven Lignans from Schisandrae chinensis Fructus by Ultra-performance Liquid Chromatography

BAI Hong-bo1,FENG Zhi-qiang2,SHI Xing-bo1,SUN Xiao-chun1,LIU Yan-ru1*,TANG Zhi-shu1*

1 Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources IndustrializationShaanxi province key laboratory of new drugs and Chinese medicine foundation research,Shaanxi University of Chinese Medicine, Xianyang 712083,China; 2Shaanxi Xingshengde Pharmaceutical Limited Liability Company,Xi’an 710119,China

Online:2017-04-29 Published:2017-05-08

摘要/Abstract

摘要： 建立了超高效液相色谱法（UPLC）同时测定北五味子中7种主要木脂素类成分的检测方法。采用ACQUITY UPLC- BEH C18色谱柱（50 mm×2.1 mm，1.7 μm）；以水-乙腈为流动相，梯度洗脱分离，PDA检测波长254 nm，流速0.4 mL/min，柱温30 ℃，进样量2 μL。优化了色谱分离条件，并进行了线性方程、准确度、重复性、回收率等方法学考察，在优化条件下，7种木脂素的出峰时间在5 min内，且线性关系良好（r>0.9994），方法检出限范围为0.0105~0.1600 μg/mL，定量限为0.1050~0.8200 μg/mL，相对标准偏差（RSD）在0.0340%~1.3800%之间，样品加样回收率在95.09%~104.50%范围内。本方法大大缩短了分析时间，灵敏度高，适用于北五味子中7种木脂素类成分的同时测定。

关键词: 北五味子, 超高效液相色谱法, 木脂素类化合物, 同时测定

Abstract: In this study,an ultra-performance liquid chromatography(UPLC)was developed for the simultaneous determination of 7 lignans from Schisandrachinensis fruits.The analytes were separated on an ACQUITY UPLC BEH C18 column(50 mm×2.1 mm,1.7 μm) with acetonitrile-water as mobile phases in gradient elution under 254 nm.The flow rate was 0.4 mL/min and column temperature was set as 30 ℃.The injection volume was 2 μL.By using the optimized method,the detection limits of the 7 lignans ranged from0.0105 to 0.1600 μg/mL,the limits of quantitation ranged from 0.1050 to 0.8200 μg/mL.The recoveries of the 7 lignans in the samples were 95.09% to 104.5% with relative standard deviation(RSD%) ranged from 0.0340% to 1.3800%.The established UPLC method for the simultaneous determination of 7 lignans can provide a more comprehensive reference for the quality control of S.chinensi.

Key words: Schisandra chinensis, ultra-high performance liquid chromatography, lignans, simultaneous determination

中图分类号:

R932

白宏博1，冯志强2，史鑫波1，孙晓春1，刘妍如1*，唐志书1*. 超高液相色谱法同时测定北五味子中7种木脂素类化合物[J]. 天然产物研究与开发, 2017, 29(4): 605-609.

BAI Hong-bo1,FENG Zhi-qiang2,SHI Xing-bo1,SUN Xiao-chun1,LIU Yan-ru1*,TANG Zhi-shu1*. Simultaneous Determination of Seven Lignans from Schisandrae chinensis Fructus by Ultra-performance Liquid Chromatography[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2017, 29(4): 605-609.

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超高液相色谱法同时测定北五味子中7种木脂素类化合物

Simultaneous Determination of Seven Lignans from Schisandrae chinensis Fructus by Ultra-performance Liquid Chromatography

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