天然产物研究与开发 ›› 2017, Vol. 29 ›› Issue (增刊1): 153-157.doi: 10.16333/j.1001-6880.2017.S.031

• 开发研究 • 上一篇    下一篇

雪山杜鹃不同部位粗多糖的抗氧化活性研究

郭肖,周绪正,王嗣涵,朱阵,张继瑜*   

  1. 甘肃省新动物药物项目重点实验室 农业部兽药研制重点实验室 中国农业科学院兰州畜牧与兽药,兰州 730050
  • 出版日期:2017-08-02 发布日期:2017-08-02

Antioxidative Activity of Crude Polysaccharides from Different Parts of Rhododendron aganniphum Balf.f.et K.Ward

GUO Xiao,ZHOU Xu-zhen,WANG Si-han,ZHU Zhen,ZHANG Ji-yu*   

  1. Key Laboratory of New Animal Drug Project of Gansu Province,Key Laboratory of Veterinary  Pharmaceutical Development of Ministry of Agriculture,Lanzhou Institute of Husbandry and Pharmaceutical  Sciences,Chinese Academy of Agricultural Sciences,Lanzhou 730050,China
  • Online:2017-08-02 Published:2017-08-02

摘要: 采用超声波辅助法提取雪山杜鹃粗多糖,考察雪山杜鹃旧叶粗多糖(RABPL2)、雪山杜鹃新叶粗多糖(RABPL1)、雪山杜鹃嫩枝粗多糖(RABPT)和雪山杜鹃花粗多糖(RABPF)对·OH、O·2和·DPPH的清除作用和Fe2+螯合作用及还原能力。结果表明,四种粗多糖均具有较显著的清除·OH、O·2、·DPPH作用和明显的Fe2+螯合作用及还原能力。RABPL2 体外抗氧化活性高于其他三种粗多糖;并且清除率与浓度之间存在明显量效关系。RABPL2是一种优良的天然抗氧化剂和自由基清除剂,具有广泛的开发前景。

关键词: 雪山杜鹃, 多糖, 抗氧化活性

Abstract: In this study,RABPL2、RABPL1、RABPT and RABPF from different parts of Rhododendron aganniphum Balf.f.et K.Ward were extracted with ultrasonic method.The in vitro antioxidant activities of the four crude polysaccharides were investigated by determining the scavenging effects on·OH.O·2,·DPPH,and chelating effects on Fe2+ and reducing ability.The results showed that all of crude polysaccharides had significant effects on scavenging ·OH,O·2,·DPPH,and obvious Fe2+ chelating and reducing ability,the in vitro antioxidant activity of RABPL2 was higher than that of other three polysaccharides,and there was a doseeffect relationship was obviously.RABPL2 is kinds of excellent natural antioxidant and free radical scavenger,and have extensive development prospect.

Key words: Rhododendron aganniphum, polysaccharides, antioxidant activity