天然产物研究与开发 ›› 2017, Vol. 29 ›› Issue (增刊1): 166-170.doi: 10.16333/j.1001-6880.2017.S.033

• 开发研究 • 上一篇    下一篇

响应面法优化金樱子黄酮成分提取工艺

刘相文,侯林,崔清华,陈智,袁琦,田景振*   

  1. 山东中医药大学药学院,济南 250355
  • 出版日期:2017-08-02 发布日期:2017-08-02

Optimization of Extraction Conditions of Rosa laevigata Michx.Flavonoids

LIU Xiang-wen ,HOU Lin,CUI Qing-hua,CHEN Zhi,YUAN Qi,TIAN Jing-zhen*   

  1. College of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,China
  • Online:2017-08-02 Published:2017-08-02

摘要: 选择酶的用量、酶解温度、酶解pH为考察因素,以甘草素、槲皮素、山奈酚、芹菜素含有量的总和为指标响应值,采用响应面法优化金樱子黄酮成分的最佳提取工艺。结果表明,金樱子黄酮成分的最佳提取工艺为酶用量为0.53 mg/mL,酶解温度为51.21 ℃,PH为4.88,在此条件下,黄酮提取量预测值为25.78 μg/5 g,验证值为25.55 μg/5 g,证明该工艺稳定、可靠。

关键词: 金樱子, 黄酮, 响应面法, HPLC

Abstract: To optimize flavonoids extraction condition .With the sum of licorice,quercetin,kaempferol,apigenin as an indicator,response surface methodology was adopted to optimize flavonoids extraction condition by taking enzymatic hydrolysis temperature,pH and dosage.nzymatic hydrolysis temperature 51.21 ℃,pH 4.88,dosage 0.53 mg/mL,under this condition,the predicted value of flavonoids extraction was 25.78 μg/5g,and the test value was 25.55 μg/5g,.the optimal extraction condition were Stable and reliable.

Key words: Rosa laevigata Michx, flavonoids, response surface methodology, HPLC