关键词: 氧化苦参碱, 肿瘤细胞, 内皮细胞, 细胞增殖, 细胞活性

Abstract: The key point is observing the effects of oxymatrine on the activity of human umbilical vein endothelial cells (ECV304) and human gastric cancer cells(SGC-7901).MTT method and growth curve were used to detect the proliferation inhibitory effect of two kinds of cells at different concentrations and different times,respectively.HE staining and transmission electron microscopy were used to observe the morphological changes of two kinds of cells.The IC50 value of oxymatrine on SGC-7901 and ECV304 were 660.07 μg/mL and 1555.17 μg/mL respectively.SGC-7901 cells ofoxymatrine group appeared significant morphological changes,such as cytoplasm concentration,nucleus getting larger,and a number of cells were in the mid and late apoptotic stage.However,the morphological changes of oxymatrine on ECV304 were not obvious,and some cells were in the early apoptotic stage.Hence,oxymatrine can inhibit the proliferation of the two kinds of cells,but to SGC-7901 cells was more obvious.The morphological changes of SGC-7901 cells were more significant than ECV304 cells at the same concentration.Oxymatrine had obvious anti-tumor activity and partial anti-angiogenesis activity,which indicated that oxymatrine appeared different sensitivity to different cells.

Key words: oxymatrine, tumor cells, endothelium cells, cell proliferation, cell activity