关键词: 黄芪百合颗粒, 指纹图谱, 毛蕊异黄酮葡萄糖苷, 橙皮苷, 芒柄花苷, 毛蕊异黄酮, 芒柄花素, 川陈皮素

Abstract: To establish the HPLC fingerprint and determine six components of Huangqi Baihe Granules (HBG),so as to provide a scientific basis for the quality evaluation.The analysis was performed on a Agilent Eclipse Plus C₁₈ column (4.6 mm×250 mm,5 μm),and the mobile phase consisted of 0.2% formic acid (A) and acetonitrile (B) with gradient elution (0-10 min,5%→10%B;10-35 min,10%→30%B;35-40 min,30%B;40-50 min,30%→60%B;50-60 min,60%→5%B) at a flow rate of 1.0 mL/min.The column temperature was set at 30  ℃ and the detection wavelengths were set at 250,260,290,and 330 nm.The fingerprint chromatography included 28 mutual peaks,and the similar degrees of 10 batches of HBG were above 0.99.Six components were identified and determined by comparison with the reference substances.They were calycosin7-O-β-D-glucopyranoside (peak 15),hesperidin (peak 18),ononin(peak 21),calycosin (peak 23),formononetin (peak 25) and nobiletin (peak 26) respectively.The established method could be used for the quality control of HBG.

Key words: Huangqi Baihe Granules, fingerprint, calycosin7-O-β-D-glucopyranoside, hesperidin, ononin, calycosin, formononetin, nobiletin