关键词: 刺五加, 多酚与黄酮, 提取, 抗氧化活性

Abstract: Using Maijishan wild Acanthopanax as raw material,the parameters of ultrasonic assisted extraction process and the antioxidant activity in vitro of total polyphenols and flavonoids were studied.The better technological conditions as follows∶ultrasonic time 40 min,ultrasonic power 600 W,ultrasonic temperature 60 °C,solid-liquid ratio 1∶35 (g ∶mL).The process parameters were determined by using single factor test,orthogonal test,and three parallel validation experiments. The average extraction amount of total polyphenols is 25.21±0.17 mg GAE/g and total flavonoids is 28.11±0.19 mg RE/g.Using synthetic Vc as the control,the antioxidant activity in vitro was studied.The results showed that the total antioxidant capacity was the largest when the extract concentration reached 300 mg/L.When the concentration of extract reached 440.00 mg/L,DPPH free radical scavenging ability was the strongest,and the scavenging rate was 94.52%±1.30%.When the concentration of extract reached 130.00 mg/L,superoxide anion radical (O₂^-·）had the strongest scavenging ability,and the scavenging rate was 86.71%±1.28%.When the concentration of extract reached 100.00mg/L,the scavenging ability of hydroxyl radical (·OH) was the strongest,and the scavenging rate was 94.23% ±1.53%.Overall,the antioxidant capacity of wild acanthopanax extract in vitro was significantly stronger than that of synthetic Vc (P< 0.05).

Key words: acanthopanax, polyphenols and flavonoids, extraction, antioxidant activity