关键词: 茯苓, 胞内多糖, 胞内三萜, 液体发酵

Abstract: Experiments were performed aimed to screen out submerged fermentation start Wolfiporia cocos strains,which should be high intracellular polysaccharide yield and triterpenoid yield.PDA enrichment plates and submerged fermentation cultures were adopted to determine the mycelium growth rate,respectively.The submerged fermentation strategy was employed to analyze the potentials of 16 W.cocos strains producing intracellular polysaccharides and intracellular triterpenoids.The data from mycelium growth rate determination assays demonstrated that there is no correlation between the growth rate of mycelium grown on PDA plate and that cultured in seed medium.And our data showed that the browning phenomenon of W.cocos could be alleviated by decreasing the initial pH value of primary seed medium to 4.0.Furthermore,AS5.137 exhibited the highest intracellular polysaccharide content,up to 377.60±0.10 mg/g,but DB displayed the highest intracellular polysaccharide yield,up to 1.01±0.13 g/L.Y1 produced the most amount of intracellular triterpenoids,and the corresponding value was 83.89±4.28 mg/g.However,Jingzhou28 displayed the highest intracellular triterpenoids,up to 136.63±26.66 mg/L.As far as on the production of intracellular polysaccharides and triterpenoids of W.cocos is concerned,the data from this study suggested that AS5.137 and DB are suitably employed as submerged fermentation start strains for producing intracellular polysaccharides,while Y1,Jingzhou28,Z(z),and Xingpinzhong as submerged fermentation start strains for producing intracellular triterpenoids.

Key words: Wolfiporia cocos, intracellular polysaccharides, intracellular triterpenoids, submerged fermentation