天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (10): 1761-1766.doi: 10.16333/j.1001-6880.2020.10.018

• 开发研究 • 上一篇    下一篇

小一点红醇提物对慢性盆腔炎大鼠药效学研究

赵丹妮1,2,3,4，何国鑫1,2,3,4，周欣1,2,3,4，龚小见1,2,3,4，程力5，刘璇5，赵超1,2,3,4*   

  1. 1贵州师范大学 贵州省山地环境信息系统与生态环境保护重点实验室；2喀斯特山地生态环境保护与资源利用协同创新中心; 3贵州师范大学 贵州省药物质量控制及评价技术工程实验室；4贵州师范大学 天然药物质量控制研究中心；5贵州中医药大学第二附属医院，贵阳 550001

  • 出版日期:2020-10-28 发布日期:2020-10-30
  • 基金资助:
    贵州省高层次创新人才培养项目（黔科合人才(2015)4033号）

Pharmacodynamics of Emilia prenanthoides DC. alcohol extract in rats with chronic pelvic inflammatory disease

ZHAO Dan-ni1,2,3,4,HE Guo-xin1,2,3,4,ZHOU Xin1,2,3,4,GONG Xiao-jian1,2,3,4,CHENG Li5,LIU Xuan5,ZHAO Chao1,2,3,4*#br#

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  1. 1Guizhou Normal University,Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment;
    2Collaborative Innovation Center for Karst Mountain Ecological Environment Protection and Resource Utilization;3 Guizhou Normal University,Guizhou Engineering Laboratory for Quality Control＆Evaluation Technology of Medicine;4 Guizhou Normal University,Research Center for Quality Control of Natural Medicine;5The Second Affiliated Hospital of Guizhou University of Chinese Medicine,Guiyang 550001，China

  • Online:2020-10-28 Published:2020-10-30

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摘要：

以慢性盆腔炎大鼠为模型，探究小一点红对其血清中炎症因子（TNF-α、IL-2、IL-6）含量、子宫肿胀程度及组织病变程度的影响。实验中采用苯酚胶浆法制备慢性盆腔炎模型大鼠，分为空白组、模型组、假手术组、妇科千金片阳性组（0.86 g/(kg·d)）、小一点红醇提物高、中、低剂量组（0.8、1.6、3.2 g/(kg·d)）。以血清中TNF-α、IL-2、IL-6含量和子宫的肿胀度来探究大鼠体内炎症情况，以模型大鼠子宫组织病变情况对病理形态学进行评价，进而考察小一点红对慢性盆腔炎大鼠的影响。结果发现，与模型组比较，小一点红醇提物可有效抑制大鼠血清内中TNF-α、IL-2、IL-6含量（P < 0.05），改善盆腔炎大鼠子宫炎症病变，减轻细胞炎症物质浸润程度，缓解组织黏连情况。上述研究表明，小一点红可有效抑制大鼠体内的相关炎症反应，对慢性盆腔炎具有显著的治疗作用，其作用机制可能与TNF-α、IL-2、IL-6含量的降低有关。

关键词: 小一点红, 慢性盆腔炎, TNF-α, IL-2, IL-6, 病理组织形态学

Abstract:

The effect of oral Emilia prenanthoides DC.(EP) in rats with chronic pelvic inflammatory disease was explored.Serum levels of inflammatory factors (TNF-α,IL-2,IL-6),uterine swelling and tissue lesions were used as evaluation indicators.Phenol gel was used to prepare chronic pelvic inflammatory disease rats which were divided into blank group,model group,sham operation group,gynecological qianjin tablet positive group (0.86 g/(kg·d)),and EP high,EP medium and EP low dose groups (0.8,1.6 and 3.2 g/(kg·d)).The contents of TNF-α, IL-2,IL-6 in the serum and the degree of swelling of the uterus were used to investigate the inflammation in the rats.The morphological evaluation of the rats was performed based on the pathological changes of the uterus influences.This was found to occur after oral administration of EP in rats,where the inflammatory cytokine TNF-α, IL-2, IL-6 was inhibited(P<0.05),the inflammatory lesions of uterus were improved,the infiltration of inflammatory substances in cells was reduced.In conclusion,the EP can effectively inhibit the related inflammatory response in rats and have a significant therapeutic effect on chronic pelvic inflammation.The mechanism may be related to the reduction of TNF-α, IL-2 and IL-6 content.

Key words: Emilia prenanthoides DC., chronic pelvic inflammatory disease, TNF-α,IL-2, IL-6, histomorphology

中图分类号:  R932

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