天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (增刊1): 136-141.doi: 10.16333/j.1001-6880.2020.S.022

• 开发研究 • 上一篇    下一篇

淀粉酶提取谷糠蛋白的工艺研究

吴兴雨1,曹阔1,姚玥1,孙丰梅1,2*   

  1. 1河北北方学院食品科学系;2河北省农产品食品质量安全分析检测重点实验室,张家口 075000

  • 出版日期:2020-06-28 发布日期:2020-07-21
  • 基金资助:
    河北省教育厅项目(ZD2017205);河北省科技厅项目(18227132D)

Study on extraction technology of bran protein by alkaline protease

WU Xing-yu1,CAO Kuo1,YAO Yue1,SUN Feng-mei1,2*   

  1. In this paper,millet bran was used as raw material to extract millet bran protein with amylase.Four factors including enzyme dosage,time,temperature and pH value were investigated,and the extraction rate of millet bran protein was optimized by single factor test and response surface methodology.The best process for obtaining millet bran protein is as follows:enzyme dosage:1.8%,extraction time:2.2 h,extraction temperature:55.6℃,pH:6.5.Under these conditions,three tests were carried out and the average value was taken to obtain the best extraction rate of 48.53%.

  • Online:2020-06-28 Published:2020-07-21

摘要:

本文以小米谷糠为原材料,采用淀粉酶提取小米谷糠蛋白,对加酶量、时间、温度和pH值四个因素进行考察,并用单因素试验和响应面法优化小米谷糠蛋白的提取率。最后得到小米谷糠蛋白的最佳工艺为:加酶量:1.8%,提取时间:2.2 h,提取温度:55.6 ℃,pH6.5,在该条件下进行三次试验,取平均值得到最佳提取率为48.53%。

关键词: 小米谷糠, 淀粉酶, 单因素试验, 响应面优化

Abstract:

本文以小米谷糠为原材料,采用淀粉酶提取小米谷糠蛋白,对加酶量、时间、温度和pH值四个因素进行考察,并用单因素试验和响应面法优化小米谷糠蛋白的提取率。最后得到小米谷糠蛋白的最佳工艺为:加酶量:1.8%,提取时间:2.2 h,提取温度:55.6 ℃,pH6.5,在该条件下进行三次试验,取平均值得到最佳提取率为48.53%。

Key words: millet bran, amylase, single factor test, response surface optimization

中图分类号:  TS209