天然产物研究与开发 ›› 2021, Vol. 33 ›› Issue (10): 1751-1757.doi: 10.16333/j.1001-6880.2021.10.016

• 开发研究 • 上一篇    下一篇

绿光条件下氮浓度对紫球藻生长和生物活性产物合成的影响

郭帅1,黄子诚1,谢点1,吴黎明1,郑明敏1,2,陈必链1,2,何勇锦1,2*   

  1. 1福建师范大学生命科学学院;2福建师范大学 工业微生物教育部工程研究中心,福州 350117
  • 出版日期:2021-10-28 发布日期:2021-10-28
  • 基金资助:
    福建省科学计划引导性项目(2020N0008);国家自然科学基金(31900092);2021年大学生创新创业训练计划(cxxl-2021477)

Effects of different nitrogen concentrations on the growth and bioactive compounds biosynthesis of Porphyridium cruentum under green light

GUO Shuai1,HUANG Zi-cheng1,XIE Dian1,WU Li-ming1,ZHENG Ming-min1,2,CHEN Bi-lian1,2,HE Yong-jin1,2*   

  1. 1College of Life Sciences,Fujian Normal University;2Engineering Research Center of Industrial Microbiology of Ministry of Education,Fujian Normal University,Fuzhou 350117,China

  • Online:2021-10-28 Published:2021-10-28

摘要: 本文以紫球藻FJ-12为藻株,在发光二极管(LED)绿光条件下,研究不同氮浓度(0、0.5、1和1.5 g/L)对紫球藻细胞的生长和生物活性产物(藻胆蛋白、胞外硫酸酯多糖和多不饱和脂肪酸)合成的影响。结果表明:在氮浓度为1 g/L的条件下,紫球藻可获得最大生物量,为3.09 g/L。藻细胞在氮浓度为1 g/L和1.5 g/L的条件下培养可合成最高的藻蓝蛋白、藻红蛋白、别藻蓝蛋白、胞外硫酸酯多糖和油脂浓度与产量。紫球藻在1.5 g/L氮浓度下可合成最大的花生四烯酸(ARA)和二十碳五烯酸(EPA)占比,但ARA和EPA产量分别与1 g/L氮浓度下的ARA和EPA产量无显著差异。综上可得,在LED绿光下,1 g/L KNO3是紫球藻最适合生长和活性物质合成的氮浓度,可实现紫球藻多联产高效协同合成藻胆蛋白、胞外硫酸酯多糖和多不饱和脂肪酸,解决紫球藻协同合成活性物质的瓶颈问题,绿光和氮浓度为规模化养殖紫球藻和开发其活性物质提供实践证据。


关键词: 紫球藻, 氮浓度, 培养, 生物活性产物

Abstract:

Using Porphyridium cruentum as the algae strain,the aim of this work was to investigate the effects of different nitrogen concentrations (0,0.5,1 and 1.5 g/L of KNO3) on the growth and bioactive compounds (phycobiliproteins,extracellular sulfate polysaccharides and polyunsaturated fatty acids) biosynthesis of P. cruentum under the light-emitting diodes (LED) green light.The results showed that P. cruentum could obtain the maximum biomass (3.09 g/L) in the nitrogen concentration of 1 g/L under the LED green light.Moreover,microalga cultivated under LED greed light at 1 g/L and 1.5 g/L KNO3 achieved the highest concentrations and productivity of phycocyanin,phycoerythrin,allophycocyanin,extracellular sulfate polysaccharide and lipid.Furthermore,P. cruentum attained the highest contents of ARA and EPA at 1.5 g/L KNO3.However,no significant difference in ARA content was found by microalga between 1 g/L KNO3 and 1.5 g/L KNO3.Based on these results,it could draw a conclusion that P. cruentum grown in 1 g/L KNO3 under LED green light exhibited the excellent growth characteristic and ability to maximally produce phycobiliproteins,extracellular sulfate polysaccharides and polyunsaturated fatty acids.This work addressed the bottleneck about the coproduction of P. cruentum-derived bioactive compounds,offering the scientific reference to cultivate P. cruentum and exploit its bioactive substances for food,feed and cosmetic in future.

Key words: Porphyridium cruentum, nitrogen concentrations, cultivation, bioactive substances

中图分类号:  Q939.97