The aim of this study was to assess the hemostatic effect of militarine,a non-polysaccharide component militarine from Bletilla striata,and to investigate its potential mechanism.The hemostatic effect of militarine was preliminarily evaluated by determining the bleeding time (BT) and clotting time (CT) of mice in physiological and pathological conditions using a tail-transection mouse model and a heparinized bleeding mouse model,respectively.Coagulation parameters,including prothrombin time (PT),thrombin time (TT),fibrinogen (FIB),and activated partial thromboplastin time (APTT) were also assessed to evaluate its effect on the coagulation function of mice.In addition,the levels of P-selectin (CD62P),thromboxane B₂ (TXB₂) and 6-keto-prostaglandin F1α (6-keto-PGF1α) were determined to explore its effects and mechanisms on the platelet system.The results showed that militarine reduced BT and CT values in a dose-dependent manner in normal and heparinized mice,displaying significant hemostatic and procoagulant effects.However,for coagulation function indices (PT,TT,APTT and FIB),militarine did not show a significant effect on normal and heparinized mice but significantly increased the level of CD62P,suggesting that it might exert its hemostatic effect by affecting the platelet system.Militarine significantly increased the levels of CD62P and TXB₂ in the plasma of heparinized mice and decreased the production of 6-keto-PGF1α,suggesting that it may exert hemostatic effect by directly promoting platelet activation and enhancing the coagulation function of platelets to accelerate blood coagulation.These findings suggest that militarine may be an effective hemostatic agent.