罗勒籽脂肪酸及其对蛋白酪氨酸磷酸酯酶1B（PTP1B）抑制作用的研究

doi:A

天然产物研究与开发 ›› 2012, Vol. 23 ›› Issue (09): 1261-.doi: A

罗勒籽脂肪酸及其对蛋白酪氨酸磷酸酯酶1B（PTP1B）抑制作用的研究

胡尔西丹•伊麻木^1,2,3，马庆苓1，阿布力米提•伊力¹，热娜•卡斯木^3*, 阿吉艾克拜尔•艾萨¹

¹中国科学院新疆理化技术研究所，乌鲁木齐 830011；²中国科学院研究生院，北京 100039；³新疆医科大学药学院，乌鲁木齐 830054

出版日期:2012-09-29 发布日期:2013-01-06
基金资助:
新疆维吾尔自治区科技基础条件平台建设项目（PT0906）, 新疆特有药用资源利用省部共建实验室(XJYS0204-2011-01).

Component and PTP1B Inhibition of Fatty Acid from Seeds of Ocimum basilicum L.

YIMAMU Huerxidan^1,2,3, MA Qing-ling¹, YILI Abulimiti¹, KASIMU Rena³, AISA Hajiakebair¹

¹Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi, 830011, China;²Graduate School of the Chinese Academy of Sciences, Beijing 100039; ³Pharmaceuticals institute of Xinjiang medical university, Urumqi, 830054, Xinjinag, China

Online:2012-09-29 Published:2013-01-06

摘要/Abstract

摘要： 利用索氏提取法提取罗勒籽油，向罗勒籽油加入氢氧化钾甲醇溶液后并用水浴加热，加入正己烷和蒸馏水萃取，上清液即为罗勒籽油中脂肪酸，用气相色谱质谱法（GC/MS）对脂肪酸进行鉴定。共鉴定出了4种脂肪酸，其中α-亚麻酸为62.88%、亚油酸为20.66%、棕榈酸为10.67%、硬脂酸为5.79%。对罗勒籽脂肪酸进行PTP1B的抑制作用研究，结果表明脂肪酸对PTP1B有较强的抑制作用，其IC50为11.12 μg/mL。该研究为深入研究罗勒籽的药理作用提供了科学依据。

关键词: 罗勒籽, 脂肪酸, 气相色谱/质谱, PTP1B抑制作用

Abstract: Fats and oil were extracted by Soxhlet extraction from the seeds of Ocimum basilicum L.. And then fats and oil were dissolved in KOH of methonal with water-bath. Lastly, the fatty acids were extracted with n-hexane. The chemical constituents of the fatty acids were identified and quantified by means of capillary gas chromatography combined gas Chromatography-mass spectrometry. The results indicated that the fatty acids included four kinds of fatty acids: α-linolenic acid (62.88%), linoleic acid (20.66%), hexadecanoic acid (10.67%) and octadecanoic acid (5.79%). The fatty acids had strong inhibiting effect on PTP1B with IC50 value of 11.12 μg/mL. The results provided the basis for further study of the seeds of O. basilicum.

Key words: Ocimum basilicum L. seeds, fatty acid, gas chromatography-mass spectrometry, PTP1B

中图分类号:

O657.63

胡尔西丹?伊麻木，马庆苓，阿布力米提?伊力，热娜?卡斯木, 阿吉艾克拜尔?艾萨. 罗勒籽脂肪酸及其对蛋白酪氨酸磷酸酯酶1B（PTP1B）抑制作用的研究[J]. 天然产物研究与开发, 2012, 23(09): 1261-.

YIMAMU Huerxidan, MA Qing-ling, YILI Abulimiti, KASIMU Rena, AISA Hajiakebair. Component and PTP1B Inhibition of Fatty Acid from Seeds of Ocimum basilicum L.[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2012, 23(09): 1261-.

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罗勒籽脂肪酸及其对蛋白酪氨酸磷酸酯酶1B（PTP1B）抑制作用的研究

Component and PTP1B Inhibition of Fatty Acid from Seeds of Ocimum basilicum L.

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