朱砂根抑制α-葡萄糖苷酶与抗氧化活性研究

doi:A

摘要/Abstract

摘要： 对朱砂根抑制α-葡萄糖苷酶与抗氧化活性进行研究。利用96微孔板法筛选α-葡萄糖苷酶抑制活性；采用DPPH、ABTS和FRAP方法分析抗氧化活性。结果表明，乙酸乙酯部位抑制α-葡萄糖苷酶的活性最高（IC50 = 39.27 μg/mL），石油醚部位次之（IC50=56.11 μg/mL），正丁醇部位活性最弱（IC50=62.05 μg/mL），但均远大于阳性对照Acarbose（IC50=1081.27 μg/mL）；乙酸乙酯部位抗氧化能力最强，正丁醇部位次之。乙酸乙酯部位清除DPPH自由基（IC50=38.55 mg/L）的能力比BHT（IC50=18.71 mg/L）低1/2，清除ABTS自由基的能力（IC50=3.60 mg/L）比BHT（IC50=7.44 mg/L）强，但比BHA（IC50=1.74 mg/L）弱，还原Fe3+的能力（FRAP=512.99±6.80 μmoTE/g）为BHT（FRAP=1581.68±97.41μmol TE/g）的1/3。结果显示朱砂根乙酸乙酯部位抑制α-葡萄糖苷酶和抗氧化活性最好。

关键词: 朱砂根；&alpha, -葡萄糖苷酶抑制活性；抗氧化活性

Abstract: To investigate the α-glucosidase inhibitory and antioxidant activity of Ardisia crenata, 96-microplate-based method was used to assay α-glucosidase inhibitory activity of A. crenata and antioxidant activity was determined by the method of DPPH, ABTS, and FRAP. The results showed that the ethyl acetate extract (IC50=39.27 μg/mL) had the highest α-glucosidase inhibitory activity, the petroleum ether extract came second (IC50=56.11 μg/mL), and the n-butanol extract was the weakest (IC50=62.05 μg/mL). But all of them showed higher activity than Acarbose (IC50=1081.27 μg/mL). The ethyl acetate extract showed the highest antioxidant activity which was higher than n-butanol extract. The DPPH radical scavenging activity of ethyl acetate extract (IC50=38.55 mg/L) was half of BHT (IC50=18.71 mg/L) while ABTS radical scavenging activity (IC50=3.60 mg/L) was higher than that of BHT (IC50=7.44 mg/L) and lower than that of BHA (IC50=1.74 mg/L). It exhibited the ferric reducing antioxidant power (FRAP=512.99 ±6.80 μmol TE/g) which was almost one third of BHT (FRAP=1581.68±97.41 μmol TE/g). The results indicated that the ethyl acetate extract of A. crenata exhibited the strongest activity of α-glucosidase inhibitory and antioxidant activities.

Key words: Ardisia crenata, α-glucosidase inhibitory activity, antioxidant activity

中图分类号:

R284.2

李园园，李锟，王俊霞，康文艺. 朱砂根抑制α-葡萄糖苷酶与抗氧化活性研究[J]. 天然产物研究与开发, 2012, 23(09): 1257-.

LI Yuan-yuan, LI Kun, WANG Jun-xia, KANG Wen-yi. α-Glucosidase Inhibitory and Antioxidant Activity of Ardisia crenata[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2012, 23(09): 1257-.

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