石榴皮多酚提取物及纯化物对α-葡萄糖苷酶的抑制作用研究

doi:A

摘要/Abstract

摘要： 采用紫外分光光度法研究了石榴皮多酚提取物及其2种纯化物（P-1 和P-2）对α-葡萄糖苷酶体外抑制作用以及纯化物对该酶的抑制作用类型。结果显示，石榴皮多酚提取物和纯化物对α-葡萄糖苷酶活性均表现出较强的抑制作用，且其作用大小与浓度呈明显的剂量-效应关系；3种多酚样品中，纯化物P-2的抑酶活性最强，纯化物P-1次之，提取物最弱，它们对α-葡萄糖苷酶的半数抑制浓度（IC50，mg/mL）分别为0.045、0.185和0.278。石榴皮多酚纯化物P-2对α-葡萄糖苷酶抑制作用类型为反竞争性抑制；浓度为0.01 mg/mL时该纯化物对α-葡萄糖苷酶的抑制常数Ki为1.22 μg/mL。

关键词: 石榴皮；多酚；&alpha, -葡萄糖苷酶；抑制作用；抑制类型

Abstract: This paper studied the inhibitory effect of a polyphenol extract from pomegranate peel and its two purified fractions (P-1 and P-2) on the activity of α-glucosidase by applying UV spectrophotometry. In addition, the inhibition type of one purified fraction was determined. The extract and the purified fractions showed a dose-dependent inhibitory effect on α-glucosidase activity, of which P-2 demonstrated the strongest inhibition followed by P-1 and the extract, their 50% inhibition concentrations (mg/mL) on the enzyme being 0.045, 0.185 and 0.278, respectively. The purified fraction P-2 exhibited an anti-competitive inhibitory effect on α-glucosidase and its inhibition constant was 1.22 μg/mL at 0.01 mg/mL.

Key words: Pomegranate peel, polyphenols, α-glucosidase, inhibitory effect, inhibitory type

中图分类号:

O629.9

张海均，贾冬英，孙慧，姚开. 石榴皮多酚提取物及纯化物对α-葡萄糖苷酶的抑制作用研究[J]. 天然产物研究与开发, 2012, 23(09): 1253-.

ZHANG Hai-jun, JIA Dong-ying*, SUN Hui, YAO Kai. Inhibitory Effect of Polyphenol Extract from Pomegranate Peel and Its Purified Fractions on α-glucosidase Activity[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2012, 23(09): 1253-.

参考文献

1 Madar Z,Omunsky Z.Inhibition of intestinal α-glucosidase activity and postpradial hyperglycemia by α-glucosidase inhibitors in fa/fa rats.Nutr Res,1991,11:1035-1046.
2 Zhao J(赵骏),Gao L(高岚).Sugar and lipid-decreasing effect of Folium Mori Polysaccharide.Tianjin J Tradit Chin Med(天津中医药杂志),2004,21:505-506.
3 Kim JS,Kwon ES,Son KH,et al.Inhibition of alpha-glucosidase and amylase by Iutnolin,a flavonoid.Biosci Biotech Biochem,2000,64:2458-2461.
4 Hu JY(胡竟一),Lei L(雷玲),Liu YO(刘亚欧),et al.Study on inhibition of α-glucosidase from mulberry.Pharm Clin Chin Mat Med(中药药理与临床),2006,22(6):44-45.
5 Lin ZC(林珠灿),Guo SH(郭素华).Study on inhibition of the polyphenol extracts from tow Chinese herbal medicines on α-glucosidase.Mod Med J China(中国现代医药杂志),2011,13(2):8-9.
6 Singh RP,Chidambara Murthy KN,Jayaprkasha GK.Studies on the antioxidant activity of pomegranate(Punica granatum) peel and seed extracts using in vitro models.J Agric Food Chem,2000,50:81-86.
7 Shen ZM(沈忠明),Li Y(李英),Jiang H(姜宏),et al.Study on inhibition of the hypoglyeemic Chinese herbal medicines on alpha-glucosidase.Chin J Biol Pharm(中国生化药物杂志),2000,21(2):69-70.
8 Sch?fer A,H?gger P.Oligomeric procyandins of French maritime pine bark extract effectively inhibit α-glucosidase.Diabetes Res Clin Pract,2006,3731-3737.
9 Yin XZ(尹学哲),Quan JS(全吉淑),An CS(安昌善),et al.Study of effect and its mechanism of green tea extract on glucose tolerance in diabetic rats.Mod J Int Tradit Chin and West Med(现代中西医结合杂志),2004,13:1145-1146.
10 Lin YH(林玉桓),Wang XL(王栩林),Wang Y(王颖),et al.Inhibition of 34 kinds of Chinese herbal medicines on α-glucosidase activity.J Dalian Ins Lig Ind(大连轻工业学院学报),2004,23:270-272.
11 Liu JC(刘杰超),Jiao ZG(焦中高),Wang SX(王思新).Inhibitory effects of apple polyphenol extracts on α-amylase and α-glucosidase.J Fruit Sci(果树学报),2011,28:553-557.
12 Kang WY(康文艺),Zhang L(张丽).α-Glucosidase inhibitory activity of extracts of five genera of gesneriaceae.Nat Prod Res Dev(天然产物研究与开发),2010,22:122-125.
13 Shang YD(尚禹东),Zhang ZY(张郑瑶),Ding YL(丁云录),et al.Inhibitory effect of ginkgo biloba extract on alpha-glucosidase and its hypoglycemic mechanism.J Jilin Univ Med Ed(吉林大学学报(医学版),2011,37:427-432.

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