大孔吸附树脂纯化海红果黄酮工艺的研究

doi:A

摘要/Abstract

摘要： 通过采用大孔吸附树脂对海红果黄酮粗提液的静态吸附和解吸试验，从10种大孔吸附树脂中筛选出海红果黄酮纯化的最优树脂，考察了该树脂对海红果黄酮的静态、动态吸附与解吸性能并对吸附与洗脱的最佳条件进行了研究。结果表明：NKA-9树脂对海红果黄酮有很好的吸附和解吸性能，其最优的动态吸附工艺条件为：上样液pH值为4.0，浓度5.15 mg/mL，上样量为4BV，流速控制在2BV/h。最优的解吸工艺条件为：洗脱剂为80%乙醇溶液，洗脱液用量为3BV，洗脱流速控制在1BV/h。在此优化条件下，海红果黄酮的吸附率、解析率、收率、纯度的平均值分别达到为(79.39±0.13)%，(84.14±0.11)%，(68.20±0.15)%和(28.81±0.06)% (n=5)。

关键词: 海红果, 黄酮, 大孔吸附树脂, 纯化

Abstract: The suitable resin was selected by static and dynamic adsorption from 10 kinds of macroporous absorbent resins to Malus micromalus Makino flavonoids. The purification technology of the crude extracts by macroporous adsorbing resins were decided through the experiments. The results showed that NKA-9 resin was selected because it gave the most effective of separation. The optimum process for the purification was as follows: the concentration of sample solution was 5.15 mg/mL, pH was 4.0, the loading weight was 4BV and the adsorption flow rate was 2 BV/h; then eluted with 80% ethanol for 3 BV with elution flow rate of 1BV/h. In these optimum conditions, average adsorption rate, desorption rate, yield and purity of the flavonoids was reached to (79.39±0.13)%, (84.14±0.11)%, (68.20±0.15)% and (28.81±0.06)% (n=5), respectively.

Key words: fruit of Malus micromalus Makino, flavonoids, macroporous adsorption resin, purification

中图分类号: R283.6

徐玉霞，王华斌. 大孔吸附树脂纯化海红果黄酮工艺的研究[J]. 天然产物研究与开发, 2012, 23(09): 1297-.

XU Yu-xia, WANG Hua-bin. Purification Technology of Malus micromalus Makino Flavonoids by Macroporous Absorbent Resins[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2012, 23(09): 1297-.

参考文献

1 Bao ZH(鲍智鸿),Hu XS(胡小松).Study on process and formula of Malus micromalus Makino tea(dried).Chin Agric Sci Bull(中国农学通报),1999,(4):34~36.
2 He XL(贺学林).The development and utilization of Malus micromalus Makino trees resources.Northern Horticulture(北方园艺),2003,1(4):32.
3 Zhao L(赵亮),Liu EL(刘恩荔),Li QS(李青山),et al.Content determination of total flavonoids in fruit of Malusmicromalus by ultraviolet spectrophotometry.J Shanxi Med Univ(山西医科大学学报).2006,37(2):169~171.
4 Liang TG(梁泰刚),Liu EL(刘恩荔),Zhao CX(赵承孝),et al.Simultaneous determ ination of four flavonoids in Malus prunifolia from Shanxi province by RP-HPLC.Chin J Chin Mater Med(中国中药杂志),2009,34(17):2217~2219.
5 Zhao L(赵亮).Preliminary study on pharmaceutical value of Malus micromalus Makino.Taiyuan:Shanxi Medical University(山西医科大学),PhD.2006.
6 He W,Li W.Application of macroporous absorbed resin in separation and purification of traditional Chinese medicine.J Nanjing Univ Trad Chin Med(南京中医药大学学报),2005,21(2):134~136.
7 He Q(何琦),Ji YQ(及元乔),Ding LS(丁立生),et al.Study on D140 macroreticular adsorbent adsorption properties for flavonoid glycosides from Ginkgo biloba L..Nat Prod Res Dev(天然产物研究与开发),2001,13(1):56~59.
8 Wang TT(王婷婷),Cao SW(曹树稳),Yu HY(余燕影),et al.Adsorption and Separation of Total Flavones from Millettla nitida var.hirsutissima by Macroporous Adsorption Resin.Nat Prod Res Dev(天然产物研究与开发),2008,20(3):477~481.
9 Li C(李超),Wang NX(王乃馨),Zheng Y(郑义),et al.Using AB-8 Macroporous Adsorption Resin for Separation and Purification of Total Flavonoids from Stenoloma chusana(L.)Ching.Food Science (食品科学),2011,32(16):31~35.
10 Song LH(宋立华),Zheng YR(郑远荣),Sun XJ(孙向军),et al.Research on the extraction of flavone from cactus by ultrasonic.Journal of Shanghai Jiao Tong University(Agricultural Science) (上海交通大学学报(农业科学版)),2008,26(1):70~73.
11 Zhang B(张斌),Jiang LK(蒋立科),Wang HM(王红梅).Study on extraction technology of total flavonoids from Leaves of Magnclia Grandiflora.Chemistry & Bioengineering(化学与生物工程),2009(5)32~34.
12 Zhang YJ(张意静).Food analysis technology.Beijing:China Light Industry Press(中国轻工业出版社),2001.
13 Li BT(李伯廷),Wang X(王湘),Li XJ(李小进).Application of macroporous absorbed resin in separation and purification of natural product.Chinese Traditional and Herbal Drugs(中草药), 1990,21(8):42~44.
14 Jing Y(景怡),Jing RQ(景荣琴),Ren Y(任远),et al.Study on the Separation and Purification the Total Flavonoids in the Stigma Maydis by AB-8 Macroporous Absorption Resins. Acta Chin Med Pharm(中医药学报),2010,38(1):75~78.
15 Liang SL(梁少玲),Cai Y(蔡宇),Yang YX(杨燕霞).Application of macroporous absorbed resin in separation and purification of flavonoids and saponin.LiShiZhen Medicine and Materia Medica Research(时珍国医国药),2006,17(2):277~278.

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