天然产物研究与开发 ›› 2012, Vol. 23 ›› Issue (1): 76-78.doi: A

• 研究简报 • 上一篇    下一篇

梨花中熊果苷的高效液相色谱分析

李 媛，乔进春，张玉星，何童森，吕明霞，张 飞，崔 同*   

  1. 河北农业大学食品科技学院，保定 071000
  • 收稿日期:2010-10-25 出版日期:2012-01-30 发布日期:2012-02-15

Quantification of Arbutin from Pear Blossoms by HPLC

LI Yuan,QIAO Jin-chun,ZHANG Yu-xing,HE Tong-sen,LV Ming-xia,ZHANG Fei,CUI Tong*   

  1. College of Food Science and Technology,Agricultural University of Hebei,Baoding 071000
  • Received:2010-10-25 Online:2012-01-30 Published:2012-02-15

PDF (PC)

481

摘要： 采用高效液相色谱法对不同时期、不同品种的梨花中熊果苷的含量进行了分析，色谱柱为Hypersil BDS C18（4.6 mm × 250 mm，5 μm），流动相为甲醇:水（6:94），加入甲酸0.05%，检测波长280 nm。结果表明：熊果苷在0.01~5.00 μg范围内线性关系良好（r = 0.9999），平均加标回收率为97.7%。不同生长期的鸭梨梨花中熊果苷的含量一般在10 mg/g以上，尤以花芽萌动期时含量最高，达到35.7 mg/g（鲜重计）。不同品种的9份梨花样品中，熊果苷含量在3.5~10.5 mg/g之间。

关键词: 梨花, 熊果苷, HPLC

Abstract: The contents of arbutin in pear blossoms of different periods and different varieties were detected using the HPLC method with Hypersil BDS C18 column (4.6 mm × 250 mm,5 μm),the mobile phase methanol :water (6:94,with 0.05% formic acid),and the detection wavelength 280 nm.The results showed that the calibration curve was linear in the range of 0.01~5.00 μg (r = 0.9999) and the average recovery was 97.7%.The content of arbutin in Yali pear blossom during the florescence was above 10 mg/g (fresh weight) in most cases,especially in blossom bud germination period,reaching a maximum of 35.7 mg/g.In the nine pear blossom samples from different varieties,the contents of arbutin were range between 3.5 mg/g to 10.5 mg/g.

Key words: pear flower, arbutin, HPLC

中图分类号: 

R284.2

版权所有 © 《天然产物研究与开发》编辑部
地址：四川天府新区群贤南街289号　邮编：610299
电话：028-85210304　QQ群：342035423　Email：trcw@clas.ac.cn　
本系统由北京玛格泰克科技发展有限公司设计开发
总访问量：　今日访问：　在线人数：