蚂蚁黑色素的提取、纯化及抗氧化活性研究

doi:A

摘要/Abstract

摘要： 对黑蚂蚁采用盐酸水解、氢氧化钠溶液提取、盐酸沉淀，以及多种有机溶剂洗涤等工艺手段提取分离、纯化得到黑蚂蚁黑色素。研究发现黑蚂蚁黑色素溶于碱性溶液，不溶于水、酸及有机溶剂；其紫外吸收光谱与红外光谱具有吲哚型黑色素典型光谱性质。利用分光光度法测定了黑蚂蚁黑色素的总抗氧化能力、羟基自由基清除能力及还原力，试验结果表明：黑蚂蚁黑色素有一定的抗氧化能力，但在试验范围内其抗氧化能力比维生素C低。

关键词: 黑蚂蚁, 黑色素, 提取, 纯化, 抗氧化

Abstract: The melanin derived from the black ants was isolated and purified by acid hydrolysis, alkaline extraction, acid deposition and organic solvents abstersion. The solubility showed that the melanin was soluble in alkaline solution, but insoluble in water, acid solution and organic solvent. It may be a type of indoles melanin from the UV-visible spectrogram and the infrared spectrogram. The total antioxidant capacity, hydroxyl radical scavenging capacity and reducing power of the melanin were measured by the spectrophotometric method. The results showed that the melanin had certain antioxidant activity, but lower than that of the vitamin C.

Key words: black ants, melanin, extraction, purification, antioxidant activity

中图分类号:

TS201.2

陶浚，乔李娜，童晶晶，李杨悦，聂少平，胡晓波. 蚂蚁黑色素的提取、纯化及抗氧化活性研究[J]. 天然产物研究与开发, 2012, 23(7): 873-.

TAO Jun, QIAO Li-na, TONG Jing-jing, LI Yang-yue, NIE Shao-ping, HU Xiao-bo. Extraction, Purification and Antioxidant Activity of Melanin from the Black Ants[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2012, 23(7): 873-.

参考文献

1 Liu Y,Simon JD.Isolation and biophysical studies of natural eumelanins:Applications of imaging technologies and ultrafast spectroscopy.Pigment Cell Res,2003,16:606-618.
2 Yuan Y(袁缨).Studies on the characteristics and role of the melanin from silky fowls.J Shenyang Agric Univ(沈阳农业大学学报),1996,27:135-138.
3 Rozanowska M,Sarna T,Land EJ,et al.Free radical scavenging properties of melanin interaction of eu-and pheo-melanin models with reducing and oxidizing radicals.Free Radic Biol Med,1999,26:518-525.
4 Wang HS(王恒山),Pan YM(潘英明),Li HY(李海云),et al.Study on extraction technology of melanin from osmanthus fragrans seeds and its antioxidant activity.J Yunan Univ(云南大学学报),2004,26(6A):55-57.
5 Yan B(杨冰).Observation on the distribution of nitric oxide synthase in the brain of Polyrhachis vicina Roger.Xian:Shanxi Normal University(陕西师范大学),PhD.2008.
6 Zhang LJ（张莲姬）.Study on the antioxidant of the loach melanin.Food Res Dev(食品研究与开发),2010,31(8):11-14.
7 Li Q(李琦),Hou LH(侯丽华),Liu X(刘鑫),et al.Identification and extraction technology of melanin from Auricularia auricular.Food Sci(食品科学),2010,31(16):87-92.
8 Li R(李荣),He XL(贺学林),Xu HD(徐怀德).Melanin isolation from Zanthoxylum bungeanum seeds and protein removal.Acta Bot Boreal-Occident Sin(西北植物学报),2008,28(12):2558-2563.
9 Hu JW(胡居吾),Xiong W(熊伟),Li XH(李雄辉),et al.Integration technology on purification of Gardenia yellow of high color value with macroporous adsorption resin and extraction of organic solvent.Nat Prod Res Dev(天然产物研究与开发),2011,23:304-308.
10 Tu YG(涂勇刚),Sun YZ(孙亚真),Tian YG(田颖刚),et al.The antioxidant activity of melanin from Taihe black-bone silky fowl in vitro.J Food Sci Biotechnol(食品与生物技术学报),2009,28(2):145-149.
11 Li FR(李发荣),Yang JX(杨建雄),Shen XT(沈小婷),et al.Antioxidative activities of aucubin in vitro.J Shanxi Normal Univ(陕西师范大学学报),2004,32:98-102.
12 Oyaizu M.Studies on products of browning reaction:antioxidative activities of products of browning reaction prepared from glucose amine.Jap J Food Nutrition,1986,44:307-315.
13 Lei M(雷敏).Studies on the bioactivities of squid ink melanin and melanin-Fe.Qingdao:Ocean University of China(中国海洋大学),PhD.2008.
14 Fu JX(付晋湘).The resource exploring of black pigment in China.Cereals Oils(粮食与油脂),2005,12:42-45.
15 Xu L(徐磊),Qian ZM(钱振明),Sun JB(孙继波),et al.Isolation,purification and structural analysis of melanin from Aureobasidium pullulans.Amino Acids Biotic Res(氨基酸和生物资源),2009,31(2):9-13.
16 Tu YG(涂勇刚),Xie MY(谢明勇),Tian YG(田颖刚),et al.Spectroscopic properties and stability of melanin extracted from taihe black-bone silky fowl.Food Sci Technol(食品科技),2008,34(5):171-175.
17 Xu LP(徐利萍).Study on Extraction,Purification and Qualities of Black Pigment from Black Sesame.Wuxi:Jiangnan University(江南大学),PhD.2006.
18 Nicolaus RA,Piattelli M,Fattorusso E.The structure of melanin and melanogenesis IV on some natural melanin.Tetrahedron,1964,20:1163-1172.

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