不同商品来源降香药材中挥发油质量研究

天然产物研究与开发 ›› 2014, Vol. 26 ›› Issue (10): 1628-1633.

不同商品来源降香药材中挥发油质量研究

徐波，陈红，牛涛，孙茜*，赵国辉

天津天士力集团，天津 300402

出版日期:2014-10-30 发布日期:2014-10-31

Study on Quality of Volatile Oil of Dalbergia odorifera from Different Origins

XU Bo,CHEN Hong,NIU Tao,SUN Qian*,ZHAO Guo-hui

Tianjin Tasly Group,Tianjin 300402,China

Online:2014-10-30 Published:2014-10-31

摘要/Abstract

摘要： 建立降香药材所含挥发油相对密度及反式苦橙油醇含量测定方法，并对不同商品来源降香药材进行质量评价。水蒸气蒸馏法提取降香挥发油，采用比重瓶法测定相对密度，气相色谱法测定反式苦橙油醇含量，采用HP5色谱柱，FID检测器，以正十四烷为内标进行测定。比重瓶法准确性［RAD（相对平均偏差）%＜5%（n=10）］、重复性（RSD 0.04%＜2%）、中间精密度［RAD%＜5%（n=15）］均良好，可替代韦氏比重秤法（用量100 mL）用于降香药材相对密度检测。气相色谱法进样精密度RSD%=0.2%，重复性RSD%=0.5%，稳定性RSD%=0.6%，线性范围为0.203~5.080 mg/mL（r=0.9995），回收率为100.5%，RSD%=1.37%（n=6）。采用比重称法测定降香药材相对密度，供试品用量少（5 mL），方法简便可行，气相色谱法简单、灵敏、准确、重线性好，适用于降香油中反式苦橙油醇含量的测定，不同商品来源降香药材出油率、相对密度、反式苦橙油醇含量差别较大，分别为1.72%~3.50%、0.8680~0.9294/20 ℃、40.4%~85.4%。

关键词: 降香, 比重瓶法, 相对密度, 气相色谱法, 反式苦橙油醇

Abstract: The objectives of this study were to develop an analytical method for the determination of the relative density of volatile oil and concentration of d-nerolidol in Dalbergia odorifera, and hence to evaluate the quality of D.odorifera from different origins.D.odorifera volatile oil was extracted by steam distillation.The relative density of volatile oil was determined by pycnometer method.Gas chromatography coupled with flame ionization detector (GCFID) was used to determine the concentration of d-nerolidol.Pycnometer method was accurate ［RAD＜5%(n=10)］ with good repeatability (RSD 0.04%＜2%) and precision ［RAD%＜5%(n=15)］.Westphal balance method was successfully substituted by pycnometer method to determinate the relative density of volatile oil in D.odorifera.GCFID method was accurate and precise with RSD%< 1%.The linear relationship within the range of 0.203~5.080 mg/mL(r=0.9995) was good.The recovery was 100.5% with RSD 1.37% (n=6).Pycnometer method was simple for the determination of the relative density of volatile oil from D.odorifera with using less amount of sample.The developed GCFID method was accurate and sensitive with good reproducibility.This method was capable to be used for the determination of volatile oil in D.odorifera.The yield of volatile oil,relative density and concentration of d-nerolidol of volatile oil from D.odorifera varied with origins.

Key words: Dalbergia odorifera, pycnometer method;relative density;gas chromatography;d-nerolidol

中图分类号:

R282.71

徐波，陈红，牛涛，孙茜*，赵国辉. 不同商品来源降香药材中挥发油质量研究[J]. 天然产物研究与开发, 2014, 26(10): 1628-1633.

XU Bo,CHEN Hong,NIU Tao,SUN Qian*,ZHAO Guo-hui. Study on Quality of Volatile Oil of Dalbergia odorifera from Different Origins[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2014, 26(10): 1628-1633.

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