天然产物研究与开发 ›› 2014, Vol. 26 ›› Issue (5): 721-725.

• 开发研究 • 上一篇    下一篇

壶瓶枣干燥预处理及提取工艺对其多糖得率的影响

张耀雷1,黄立新1,2*,张彩虹1,谢普军1,游 凤1   

  1. 1 中国林业科学研究院林产化学工业研究所;生物质化学利用国家工程实验室;国家林业局林产化学工程重点开放性实验室;江苏省生物质能源与材料重点实验室,南京 210042;2 中国林业科学院林业新技术研究所,北京 100091
  • 出版日期:2014-05-31 发布日期:2014-11-17

Effects of Drying Pretreatments and Extraction Method on the Polysaccharides Yield of Ziziphus jujube Mill.cv.Hupingzao

ZHANG Yao-lei1, HUANG Li-xin1,2*, ZHANG Cai-hong1, XIE Pu-jun1, YOU Feng1   

  1. 1 Institute of Chemical Industry of Forest Products,CAF,National Engineering Lab for Biomass Chemical Utilization,Key and Open lab on Forest Chemical Engineering,SFA;Key Lab of Biomass Energy and Material,Jiangsu Province,Nanjing 210042, China; 2 Institute of New Technology of Forestry,CAF,Beijing 100091, China
  • Online:2014-05-31 Published:2014-11-17

摘要: 本文考察了不同的干燥预处理手段(烘箱干燥和真空冷冻干燥)及减压内部沸腾法中各因素(真空度、温度、液料比和提取时间等)对壶瓶枣多糖及蛋白质得率的影响。实验结果表明:干燥方式对原料枣粉的色差和多糖、蛋白质含量均有显著影响,对含水量无明显影响;真空冷冻干燥所得的枣粉色差小、多糖含量高、蛋白质含量低,综合考虑,选用真空冷冻干燥作为原料预处理手段;减压内部沸腾法最佳工艺参数为体系内温度60 ℃,液料比50:1(mL:g),提取时间30 min,此时外界温度为71 ℃,真空度为80 kPa,此条件下多糖及蛋白质得率分别为26.05 mg/g和2.14 mg/g;与传统热浸提相比,有效物质多糖得率提高了16.66%。

关键词: 壶瓶枣, 干燥, 减压内部沸腾法, 多糖, 蛋白质

Abstract: The effects of drying pretreatments (oven drying and vacuum freeze drying) and factors of reduced pressure boiling method (vacuum degree, temperature, water/solid ratio, extraction time, et al.) on the polysaccharides and protein yield of Ziziphus jujube Mill.cv.Hupingzao were investigated in this study.The result showed that drying methods had significant effects on color difference of jujube powder and its polysaccharides and protein yields,but little effect on moisture content.With comprehensive consideration,vacuum freeze drying was a better method for jujube drying pretreatment with lower color difference and protein yield as well as higher polysaccharides yield.The optimum extraction conditions of reduced pressure boiling method were:system temperature of 60 ℃,water/solid ratio of 50:1 (mL:g),time of 30 min,vacuum degree of 80 kPa,external temperature of 71 ℃.Under these conditions,the protein and polysaccharides yields were 2.14 mg/g and 26.05 mg/g,respectively.Compared with traditional water extraction,the polysaccharides yield was improved by 16.66%.

Key words: Ziziphus jujube Mill.cv.Hupingzao, drying, reduced pressure boiling method, polysaccharides, protein

中图分类号: 

TS201.2