天然产物研究与开发 ›› 2014, Vol. 26 ›› Issue (5): 738-744.

• 开发研究 • 上一篇    下一篇

响应面优化超声辅助提取香菇多糖的工艺研究

陆小雨,丁 昱,王俐人,王珊珊,郭 磊,丁之恩*   

  1. 安徽农业大学茶与食品科技学院,合肥 230036
  • 出版日期:2014-05-31 发布日期:2014-11-17

Optimization of Ultrasonic-Assisted Extraction of Lentinan by Response Surface Methodology

LU Xiao-yu, Ding Yu, WANG Li-ren, WANG Shan-shan, GUO Lei, DING Zhi-en*   

  1. School of Science and Technology of Tea and Food,Anhui agricultural University,Hefei 230036, China
  • Online:2014-05-31 Published:2014-11-17

摘要: 为确定香菇多糖的最佳提取工艺,利用响应面分析法对香菇多糖的提取工艺进行优化。在单因素实验的基础上,以超声时间、超声功率、浸提温度和浸提时间为响应因素,多糖提取得率为响应值,根据正交旋转组合试验设计原理进行四因素三水平的响应面分析。实验结果表明,采用超声功率174.94 W,超声时间为18.94 min,在80.71 ℃下提取3.01 h,得到的香菇多糖提取率最高,为9.61%。当香菇多糖的浓度为3 mg/mL,其·OH清除率为52.1%。

关键词: 香菇多糖, 超声, 提取, 响应面

Abstract: In this study,Response Surface Methodology (RSM) was applied to optimize the extraction of lentinan.On the basis of the previous one-factor test results,ultrasonic power,ultrasonic time,extraction temperature,extraction time were selected as influencing factors and the extraction yield of lentinan was used as the response value in RSM.According to the orthogonal rotation combination experiment design,the method of response with four factors and three levels was adopted.The results showed that the optimal extraction conditions were as followed:in 174.94 W of ultrasonic power,ultrasonic time 18.94 min,in 80.71 ℃ extract 3.01 h,the highest extraction yield reached at 9.61%.When the concentration of lentinan was 3 mg/mL,the ·OH scavenging rate was determined to be 52.1%.

Key words: lentinan, ultrasonic, extraction, response surface

中图分类号: 

TQ281