天然产物研究与开发 ›› 2014, Vol. 26 ›› Issue (7): 1004-1008.

• 研究论文 • 上一篇    下一篇

温度诱导双水相提取分离白藜芦醇苷的研究

傅海珍,陶青兰,杨 蕾,张 艳,戚雪勇*   

  1. 江苏大学药学院,镇江 212013
  • 出版日期:2014-07-31 发布日期:2014-11-09

Extraction and Separation of Polydatin by Temperature-Induced Aqueous Two-Phase System

FU Hai-zhen,TAO Qing-lan,YANG Lei,ZHANG Yan,QI Xue-yong*   

  1. School of Pharmacy,Jiangsu University,Zhenjiang 212013,China
  • Online:2014-07-31 Published:2014-11-09

摘要: 建立一种新的提取分离虎杖中白藜芦醇苷的方法。以具有温敏性的PE6400为提取溶剂,用超声波辅助提取白藜芦醇苷,考察了PE6400浓度、提取时间、提取次数、固液比等对白藜芦醇苷提取率的影响,其最佳工艺为25倍8%PE6400超声提取二次,每次提取30 min,白藜芦醇苷的提取率达到17.74 mg/g;在富集分离时,考察不同pH、料液比对白藜芦醇苷在温度诱导双水相体系中分配行为的影响,在料液比为1:11,pH为12的缓冲溶液,90 ℃水浴下加热45 min,83.3%白藜芦醇苷富集分离于水相中。本实验方法操作方便,提取率较高,对环境和人体毒害低,是一种便捷、高效的提取分离方法。

关键词: 白藜芦醇苷, 温度诱导, 双水相, 高效液相色谱法

Abstract: A new method for the extraction and separation of polydatin from Polygonum cuspidatum was developed.Polydatin was extracted by ultrasonic extraction with temperature-sensitive PE6400 as extraction solvent.The effects of the concentration of PE6400,extraction duration,times of extraction and solid-liquid ratio on the extraction yield of polydatin were investigated.The optimal extraction conditions were 8% PE6400 as extraction solvent,1:25 as solid-liquid ratio and ultrasonic extraction for 2 times,each time for 30 min.The extraction yield of polydatin reached 17.74 mg/g under the optimized conditions.In the process of enrichment and separation,the allocation behavior of polydatin in the temperature-induced aqueous two-phase system were also tested under different pHs,different solid-liquid ratios.When the solid-liquid ratio was 1:11,the pH of buffer solution was 12 and heated at 90 ℃water bath for 45 min,83.3% of polydatin was allocated in the aqueous phase.The developed method was convenient,efficient and easy to operate.It had high extraction rate and low toxicity to the environment.

Key words: polydatin, temperature-induced, aqueous two-phase, HPLC

中图分类号: 

R284.2