关键词: 药用白菊花, 绿原酸, 木犀草素, 芹菜素, 金合欢素, 高效液相色谱法

Abstract: A sensitive and specific high performance liquid chromatography (HPLC) method was developed to determine chlorogenic acid,luteolin,apigenin and acacetin in medicinal Chrysanthemum morfolium. The C.morfolium sample was extracted by ionic liquid based dispersive liquid phase microextraction.The chromatographic separation was carried out on a Phenomenex C₁₈ column (250 mm×4.6 mm,5 μm) with methanol-0.2% acetic acid (65:35,v:v) as mobile phases at a flow rate of 0.8 mL/min.The UV detection wavelength was set at 350 nm,and the column temperature was set at 35 ℃.The linear ranges were 0.0005~100 μg/mL for chlorogenic acid (r=0.9999),0.00095~190 μg/mL for luteolin (r=0.9999),0.0008~160 μg/mL for apigenin (r=0.9997) and 0.00061~122 μg/mL for acacetin (r=0.9996), respectively; The recoveries of chlorogenic acid,luteolin,apigenin and acacetin were 90.52%~100.44%,90.88%~98.58%,94.40%~98.82%,93.68%~100.54%, respectively.The developed HPLC method was quick,simple and reproducible for the simultaneous determination of chlorogenic acid,luteolin,apigenin and acacetin in medicinal Chrysanthemum morifolium.

Key words: medicinal Chrysanthemum morifolium, chlorogenic acid, luteolin, apigenin, acacetin, HPLC