天然产物研究与开发 ›› 2014, Vol. 26 ›› Issue (Suppl1): 105-109.

• 开发研究 • 上一篇    下一篇

云杉总黄酮抗氧化活性研究

郭霏霏1,刘文丛2*,郑毅男1*   

  1. 1 吉林农业大学中药材学院;2 吉林农业大学资源与环境学院,长春 130118
  • 出版日期:2014-12-30 发布日期:2015-03-04

Antioxidant Activity of Total Flavanoids in Picea asperata Mast.

GUO Fei-fei1, LIU Wen-cong2*, ZHENG Yi-nan1*   

  1. 1 Institute of Traditional Chinese Medicines,Jilin Agricultural University;2 College of Resources and Environment,Jilin Agricultural University,Changchun 130118,China
  • Online:2014-12-30 Published:2015-03-04

摘要: 本试验测定云杉总黄酮及其二氢槲皮素的含量,研究其抗氧化活性,为后期云杉黄酮的进一步研究提供部分理论依据。采用亚硝酸钠硝酸铝比色法测定总黄酮的含量,采用高效液相色谱法测定云杉总黄酮中二氢槲皮素的含量。采用DPPH、ABTS及还原力法测定云杉总黄酮的抗氧化能力。云杉总黄酮的含量为2.78 g/100 g,其二氢槲皮素的含量为0.374%。云杉总黄酮对DPPH、ABTS自由基有较好的清除作用,当浓度为0.5 mg/mL时清除率分别为78.7%和82.3%。其还原力也较强。

关键词: 云杉, 总黄酮, 二氢槲皮素, 含量测定, 抗氧化

Abstract: The purpose of this study was to determine the total flavonoids and dihydroquercetin contents in Picea asperata Mast.,and to further investigate its antioxidant activity.The sodium nitrite-aluminum nitrate colorimetric method and HPLC method were used to determine the contents of total flavonoids and dihydroquercetin, respectively.The DPPH scavenging,ABTS scavenging and reducing power assays were used to determine antioxidant ability of the total flavonoids of P.asperata. As the result,the total flavonoids content in P.asperata was 2.78 g/100 g,in which the dihydroquercetin content was 0.374%.In addition,the total flavonoids of P.asperata showed an obvious eliminating effect against DPPH and ABTS free radicals,at the concentration level of 0.5 mg/mL.The eliminating rate was 78.7% and 82.3%, respectively. Its reducing power was also strong.

Key words: Picea asperata Mast., total flavonoids, dihydroquercetin, content determination, antioxidant

中图分类号: 

R965.2