天然产物研究与开发 ›› 2019, Vol. 31 ›› Issue (8): 1439-1446.doi: 10.16333/j.1001-6880.2019.8.020

• 开发研究 • 上一篇    下一篇

秦皮甲素对AGEs致损的ECV304细胞增殖及氧化应激的影响

李甘1,夏大静2*   

  1. 1楚雄州人民医院输血科,楚雄 675000;2浙江大学公共卫生学院,杭州 310058
  • 出版日期:2019-09-10 发布日期:2019-09-10
  • 基金资助:

    浙江大学公共卫生学院卫健专项

Effects of esculin proliferation and oxidative stress of ECV304 cells damaged by AGEs

LI Gan1,XIA Da-jing2*   

  1. 1Department of Blood Transfusion,Chuxiong People′s Hospital,Chuxiong,675000,China; 2School of Public Health,Zhejiang University,Hangzhou 310058,China
  • Online:2019-09-10 Published:2019-09-10

摘要: 为研究秦皮甲素对血管内皮细胞的保护作用,采用CCK-8法观察秦皮甲素对体外AGEs培养的人脐静脉内皮细胞增殖的影响。检测不同浓度AGEs以及秦皮甲素作用后对内皮细胞一氧化氮(NO)、不对称二甲基精氨酸(ADMA)水平的影响以及内皮细胞氧化应激有关指标:活性氧簇(reactive oxygen species,ROS)、丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD);脂肪代谢相关指标:乳酸脱氢酶(lactic dehydrogenase,LDH)、总胆固醇(total cholesterol,CHO)、甘油三酯(triglyceride,TG)和低密度脂蛋白(low density lipoprotein,LDL),同时分别检测粘附相关因子:血管细胞粘附分子-1(VCAM-1)和细胞间粘附分子-1(ICAM-1)的表达水平。结果显示200 mg/L AGEs对人内皮细胞ECV304增殖有显著抑制作用,秦皮甲素可对抗AGEs导致的内皮细胞增殖抑制,并呈浓度依赖性。在25 mg/L时,保护效应达到最高。秦皮甲素可抵抗ROS生成。同时可改善细胞的脂类代谢:胆固醇、LDL以及TG含量在秦皮甲素作用后改善明显。秦皮甲素可显著抑制内皮粘附因子VCAM-1的表达。秦皮甲素还可上调NO水平,下调ADMA水平。总之,秦皮甲素可有效促进人血管内皮细胞增殖并在改善氧化应激,脂代谢,粘附因子和NO释放等方面发挥作用。

关键词: 秦皮甲素, 人脐静脉内皮细胞, 糖基化终末产物, 增殖作用, 氧化应激, 粘附因子

Abstract: To explore the protective effects of esculin on vascular endothelial cell line,the effect of esculin on the proliferation of human umbilical vein endothelial cells cultured in vitro by AGEs was evaluated by CCK-8 assay.The effects of different concentrations of AGEs and esculinon on the levels of nitric oxide (NO) and asymmetric dimethylarginine (ADMA) in endothelial cells were examined.Endothelial cells oxidative stress related indicators were detected,including superoxide dismutase detection (SOD),reactive oxygen species (ROS),and malondialdehyde (MDA).Lactic dehydrogenase (LDH),total cholesterol (CHO),triglyceride (TG) and low density lipoprotein (LDL) were also detected.The levels of adhesion-related factors:vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) were also evaluated.The result indicated that 200 mg/L AGEs significantly inhibited the proliferation of human endothelial cell ECV304.The esculinon could inhibit the proliferation of endothelial cells induced by AGEs,and it was concentration-dependent.At 25 mg/L,the protective effect was the highest.The oxidative stress index ROS results suggested that esculinon could resist AGEs-induced ROS production and improve liquid metabolism:cholesterol,LDL and TG levels were significantly improved after the treatment with esculinon.The esculinon could significantly inhibit the expression of the endothelial adhesion factor VCAM-1.The expression of NO was increased and the level of ADMA was deceased.Esculinon could effectively promote the proliferation of human vascular endothelial cell and had potential effects on endothelial cells in improving ROS,lipid metabolism and adhesion factors and NO releasing.

Key words: esculin, human umbilical vein endothelial cells, glycation end products, proliferation, oxidative stress, adhesion factor

中图分类号: 

R931